1897] 



MICROSCOPICAL JOURNAL. 



153 



bearing bacillus which has not infrequently appeared m 

 media boiled for ten minutes on three consecutive days. 



STERILIZATION OF BOUILLON WITH ONE BOILING. 



STERILIZATION OF AGAR WITH ONE BOILING. 



STERILIZATION OF TUBES OF AGAR CDNTAINING. A LAR- 

 GER QUANTITY FOR MAKING PLATE CULTURES. 



3 

 o 

 B 

 -< 



12 c. c , 30 mm. 



15 c. c I 30 min.. 



15 c. c 40 min. 



18 c. C 30 min. 



18 c. c 30 rain. 



18 c c 30 min. 



c O 3 2 



Remarks. 



Left at room temp, for 10 days 



Spore bearing bacillus be- 

 longing to the B. sublilit 

 group. 



