1897] MICROSCOPICAL JOURNAL. 185 



I. Fuchsin pulv, 15 grains ; Alcohol, 2 drams; Aquae 

 distillat, 1 ounce. 



II. AquaB distillat, 1 ounce ; Liquor ammonia, 3 

 minims. 



III. Alcohol, IJ ounces; iVlusp distillat, 6 drams ; 

 Nitric acid, J dram. Aniline green, to saturation. 



To stain : 



1. Grently press a small part of the most solid portion 

 of the suspected sputum between two cover glasses. 



2. During five minutes, place one cover glass in 

 equal portions of solutions one and two, heated till va- 

 por rises, 



3. Rinse in water, put a drop of solution three on it, 

 rinse again. If the mount is not a distinct green, put 

 on another drop of solution three, wash again, dry and 

 examine. 



The use of the following will also afford gratifying 

 results : 



Ziehl's Solution. — Fuchsin pulv, I'part; Alcohol, 10 

 parts; Acid carbolic, 5 per cent, sol., 100 parts. 



Grabbet's Solution. — Methylin blue, 2 parts; Acid sul- 

 phuric, 25 per cent sol., 100 parts. 



1. Prepare mount as above, hold high over a flame 

 until dry. 



2. Place cover-glass in Ziehl's solution five minutes. 



3. Place cover-glass in Gabbet's solution one minute. 



4. Dry, examine with oil immersion. 



If a hurried diagnosis is unimportant, but permanent 

 mounts desired : 



1. Place cover-glass, with dried sputum, in Ziehl's 

 solution twelve hours. 



2. Hold in nitric acid, 25 i)er cent solution, till 

 brownish black. 



3. Hold in alcohol five seconds. 



4. Hold in water one second. 



