1900] MICROSCOPICAL JOURNAL. 95 



teen to thirty minutes in 75 per cent,then the same length 

 of time in 95 per cent alcohol. From this point to paraf- 

 fine the process is the usual paraffine method. 



The tissues of the digestive system come out from this 

 solution in the most perfect condition of any of the tis- 

 sues of the body, though as before stated, the nerve fi- 

 bres of the spinal cord are the only tissues which seem 

 to be injured. 



In fact I can see no difference in the condition of tis- 

 sues thus treated and mounts of other tissues in my pos- 

 session which have been subjected to other fixing solutions. 

 One of the best results of the use of this solution is that 

 tissues thus treated can be subjected to almost any stain 

 desired with equally good effect. 



The accompanying illustrations are from photo-micro- 

 graphs taken for me by Dr. L. A. Reed of Jacksonville, 

 111. Dr. Reed has been experimenting with the fixing so- 

 lution composed of formaldehyde, mercuric chloride and 

 acetic acid ; his results exactly coincide with mine, prov- 

 ing that the absence of mercuric chloride does not affect 

 the tissues, or, in other words, that formaldehyde is an 

 excellent fixing agent. 



Figures 1 and 2 are taken from sections of the spinal 

 cord of a cat, showing the cells within the grey matter. 

 The cut does not show the effect upon the fibres, but the 

 excellent condition of the cells is apparent. 



Figures 3 and 4 are from sections of the sub-maxil- 

 lary gland of a cat. This, while not quite so clear as figs. 

 1 and 2 nevertheless shows how well the cells have been 

 fixed. 



Figures 5 and 6 are from sections of the mucous mem- 

 brane, 5 of the stomach, showing the glands of the fun- 

 dus ; and 6, of the small intestine. Figure 5 is less clear 

 than any of the others, but a close examination will show 

 that here also the cells are in an excellent condition. 



Photographs could have been made of other tissue* in 



