1888.] MICROSCOPICAL JOURNAL. 195 



He thought there was no society composed so largely of professional men, 

 nor having such an esprit de corps. He said Microscopy had so grown in 

 this decade as to make parts of the first volume of proceedings now seem 

 like boyish talk. 



Prof. W. H. Seaman presented resolutions of thanks, which w^ere heartily 

 seconded by the society. They mentioned the kindness of the Mayor and 

 municipality of Newark, of the State Microscopical Society of Ohio, of the 

 ladies of Columbus, and of the vocalists and instrumentalists who furnished 

 music the first evening. 



Professor Kellicott thanked the society for the presidential honors he had 

 enjoyed, and surrendered the chair to Dr. Wm. J. Lewis. The latter paid 

 his respects to the society, and pronounced the meeting adjourned. 



After another hour in social enjoyment the visitors took the return train 

 for Columbus, where they arrived somewhat past midnight. They then 

 scattered not to reassemble until 18S9. 



A Starch Injection Mass.* 



As PREPARED BY Prof. S. H. GAGE, 

 :thaca, n. y. 



A coarse injection mass, which is cold-flowing, may be forced nearly to the 

 capillaries, rapidly hardens after injection, leaves the vessels flexible, does 

 not dull dissecting instruments, is suitable for permanent dry or alcoholic prep- 

 aration, is simple in its manipulation, cleanly and economical, seems to be 

 fully realized in the starch mass introduced by Ad. Pansch of Kiel. 



MASS FOR ORDINARY INJECTIONS. 



Dry starch ('laundry' is good)', 100 c.c. 



Water or a 2.\ per cent, aqueous solution of chloral hydrate, f 100 c.c. 



95 per cent, alcohol, J . 35 c.c. 



Color mixture, see below, 25 c.c. 



After thoroughly mixing the mass it should be filtered through one or two 

 thicknesses of moistened paper cambric or cheese cloth. To prevent the starch 

 from settling, the cloth should be tilted from side to side or the mass may be 

 stirred during the filtration. If the mass is not freshly prepared for every in- 

 jection, the stock mass should be filtered occasionally to remove hair or any 

 other object that might clog the cannula. 



Since almost any animal injected may afford some organ worth preserving, 

 it seems better to employ permanent colors for tingeing the mass. Among 

 those which are available, probably vermilion, red lead, ultramarine, Berlin 

 blue, chrome orange, yellow, or green, are preferable. 



PREPARATION OF THE COLOR. 



Dry color, § 100 c.c. 



Glycerin, 100 c.c. 



95 per cent, alcohol, 100 c.c. 



* Since many of our readers may noi have met with this method for injection for coarse anatomy, we introduce 

 it here from the iV. Y. Medical jfournal. It is especially useful for the laboratory, because no heat is required 

 in using the mass as is the case with the gelatin fluids, and because it does not set rapidly as does the plaster. 



tThe chloral and alcohol prevent fermentation in the mass when it is kept in stock ; the alcohol also increases 

 the fluidify and likewise the more rapid hardening in the vessels ; both, of course, act as preservatives upon the 

 animal injected. 



t The mass originally recommended by Pansch consisted of wheat-flour and cold water, to which was added a 

 sufficient quantity of the desired coloring matter. Later experiments have shown that pure starch is better than 

 flour. As starch is insoluble in alcohol and cold water, it becomes hard when injected into the blood-vessels sim- 

 ply by the exudation of the liquid with which it is mixed. That the starch grains forming the mass remain en- 

 tirely unchanged may be easily demonstrated by making a microscopic examination of the contents of an injected 

 vessel. 



§ If Berlin blue is used to stain the starch, 25 c.c. of the dry blue are dissolved in 100 c.c. of water or a 5 per 

 cent, aqueous solution of chloral hydrate. 



