1885.] 



MICROSCOPICAL JOURNAL. 



131 



The Gundlacli Optical Company 

 of Rochester N. Y., are now makinj^,- 

 their microscope objectives after the 

 new principle discovered by Mr. 

 Gundlach. and described by him in a 

 paper read liefore the American So- 

 cietv of Microscopists, at tlie meeting 

 at Rochester hist Angust. Their 

 water - immersion , glycerin - immer- 

 sion, and dry objectives made upon 

 this plan are especially well spoken 

 of. With a yV glycerin objective a 

 well-known microscopist of Ohio has 

 succeeded in clearly resolving ylw/// /- 

 pleura pellucida^ in balsam, with 

 simple mirror illumination, without 

 anv accessorv apparatus. The same 

 gentleman with a \ dry objective has 

 resolved A. pcUncida in a medium 

 of 2.42 refractive index. 



The water-immersion objectives 

 have a very long working distance. 

 and the observations of higher order 

 are corrected to a much higher de- 

 gree than was heretofore possible in 

 a water-immersion objective ; hence 

 these objectives have a definition and 

 resolving power found in oil-immer- 

 sion objectives only. This series of 

 objectives may therefore be regarded 

 as a new improvement hi the field of 

 microscopic apparatus, a water-im- 

 merson objective of highest optical 

 quality having also a long working 

 distance. The objectives are pro- 

 vided with collar adjustment. 

 o 



Staining Tissues in Microscopy.* 

 III. 



BY PROF, HANS GIERKE. 



[ Continued from p. loy. ] 

 AMMONIUM MOLYBDATE. 



49. Merkel. Von Henle in seinem 



Handbuch der Nervenlehre des 



Menschen. Braunschweig, 



1871. Band III d. Handbuch 



d. Anat. d. Menschen mitge- 



theilt. 



Dilute I part saturated solution of 



ammonium molybdate with 1-2 parts 



water, add a pinch of limatura ferri. 



* From the Zeitschrift fijr wissensschafiliche Mikros- 

 kopie. Translatedfor this Journal by Prof. Wm. H. 

 Seaman, M. D. 



Add drop by drop, with constant 

 stirring, suflicient hydrochloric acid 

 to produce a dark blue, nearlv black. 

 The cloudy white precipitate formed 

 at first soon dissolves by stirring, but 

 if the solution turns brown it is worth- 

 less. After ten minutes filter. It 

 will be found particularly adapted for 

 nerves, sections of which stain in from 

 6 to 15 hours. 



50. Krause. 



In various hand-books, as Frey 7th 

 ed., Thanhoftbr, and Dippel, Krause 

 is named as discoverer of a method 

 of staining with ammonium molyl)- 

 date. He stains dark blue in about 

 24 hours with a 5% solution in water. 

 Stainings may be made brown by 

 subsequent treatment with 1% sol. 

 tannic acid or 20% pyrogallic acid. 

 The stain is recommended for nerves, 

 glands, and ciliated cells. 



MADDER DYES. 



5i. Lieberkuhn. Miiller's Archiv. 

 1854 u. Ueb. d. Wachsthum 

 desUnterkiefers u. der Wirl:)el. 

 Sitzber. d. Ges. z. Beforde- 

 rung der ges. Naturwiss. 

 Marbg., 1867, No. 10. 

 Living animals were fed with mad- 

 der to study the formation of bone, 

 the dye uniting with the forming bony 

 matter. 



52. Kolliker. Die normale Resorp- 



tion des Knochengewebes. 

 Leipzig, 1873. 

 Same process as 51 ante. 



53. Lieberkuhn. (i) Ueber die Ein- 



wirkung von Alizarin auf die 

 Gewebedes lebenden Korpers. 

 Marburger Sitzungsber, 1874, 

 p. 33, u. (2) Ueber das Ver- 

 halten des Alizarin (1. c. 



P- ID- 



Atter feeding pigeons on madder 

 the dye unites with the lime salts of 

 the bones, but not with the organic 

 matter. The latter may be removed 

 by boiling in soda solution without 

 injury to the dye. By injecting a 5% 

 solution of sodium-alizarin into dogs, 

 stainings were obtained — in young 

 dogs the entii'e bones, in old ones 

 the inner surface only, became red. 



