1885.] 



MICROSCOPICAL JOURNAL. 



211 



97. V. Thanhofter. Ueber die Entz- 

 ' iindung nebst einigen Bemer- 



kungen iiber die Structur der 

 Hoinhaut und iiber die Eosin 

 Reaction. Centiabl. d. med. 

 Wiss. 1877, No. 49, p. 881. 

 Eosin was used in investigating 

 blood corpuscles and blood vessels. 

 (See No. 87). Treatment by a one 

 per cent, solution of perosmic acid 

 for a few seconds or a minute before 

 staining, intensified the color and 

 made the preparation more perma- 

 nent. (This modification of Wis- 

 sowzky's method is very much to be 

 recommended. I have found best a 

 3 minute dip in a ^ per cent, osmic 

 acid bath, then wash v\'ell and put in 

 the eosin-alum-alcohol of No. 87). 



98. Cech C. O. Eosin als Tinctions- 



mittel. Zeitschr. f. Mikrosk. 

 h P; 65-73. 

 Eosine is recommended for stain- 

 ing. 



99. Renaut. Applications des pro- 



prietes elective de I'eosine 

 soluble dans I'eau a I'etude 

 du tissu conjonctiv. Arch, de 

 Phys., 1877, 2 Ser., iv, p, 21 1- 



243- 

 Sections are treated J- to i minute 



by an aqueous solution of eosin to 

 which ^ part of alcohol is sometimes 

 added, then washed in distilled wa- 

 ter and preserved in glycerin con- 

 taining one per cent, of salt. The 

 latter is required to counteract the 

 solubility of eosin in glycerin. Pro- 

 toplasm takes the dye readily. In 

 investigating subcutaneous connec- 

 tive tissue, a solution of one part 

 eosin to 500 water may be injected. 

 Elastic fibers color strongly, granu- 

 lated protoplasm masses show an 

 intensely red nucleus, fixed cells be- 

 come rose color, and the bundles of 

 fibrillae and striated tissue remain 

 colorless. Tactile corpuscles are no 

 darker than protoplasm. Cartilage 

 cells show dark nucleolar granules, 

 but the cartilage proper or funda- 

 mental tissue does- not stain. The 

 nuclei of the endothelium, those 

 found between Ranvier's cords and 



those belonging to Remak's fibres, all 

 stain more intensely than their sur- 

 roundings. 



100. Erlicki. Sur les moyens de 



durcir et de colorer les tissus 

 de centres nerveux. Progres 

 med. 1877. Sep., 39. Re- 

 vue des vSc. med., XI, 13; 

 Warschauer med. Zeitschrift. 

 xxiii, No. 15 und 18. 

 Erlicki used green methyl anilin in 

 his examination of the large nerves. 

 He made a 2^ per cent, aqueous so- 

 lution and left sections 24 hours 

 therein. The nuclei of the neuro- 

 glia became green, while the axis 

 and ganglia cells remained uncolored. 

 (The dye I have used with the above 

 name does not produce such difier- 

 entiation) . 



1 01. Weigert. Bismarckbraun als 

 Farbemittel. Arch. mikr. Anat. 

 XV, 25S-260. 1878. 



Bismaixk brown, an anilin dye, is 

 recommended for microscopic work 

 and preferred to carmine, picrocar- 

 mine, and eosin. Weigert names the 

 following properties of a good dye. 

 It must stain with certainty, quickly 

 and not in excess, and should bear 

 long washing without extraction, 

 must be permanent and capable of 

 preservation in mediums not too 

 highly refractive. The Bismarck 

 brown of the Berliner Actiongesell- 

 schaft fiir xA-nilinfarbenfabrication has 

 these qualities in a higher degree 

 than any other stain. A concentrated 

 solution in water, or a weak alcholic 

 solution is employed. The first is 

 prepared by boiling the dye in dis- 

 tilled water and filtering. Material 

 hardened in alcohol or chromis 

 acid stains equally well. The stain- 

 ing may be done in a few minr 

 utes, but no injury occurs if the 

 sections lie in the dye some time. 

 Mounting is done in Canada balsam 

 after washing in absolute alcohol, 

 or if glycerin is used preferably 

 in distilled water. The nuclei stain 

 most deeply , protoplasmic masses and 

 connective tissue light yellow. Amy- 

 loids are not clearlv differentiated. 



