L885.] 



MICROSCOriCAL JOURNAL. 



213 



cause evervthino- becomes a unifonn 

 red. 



(Safranin, as a reag-ent for aniyloid. 

 is much inferior to iodine violet, 

 methyl green, or Leonard's ink (So). 

 and cannot be recomniended.) 

 los- Kvber. Weitere untersuchim- 

 gen iiber die amyloid Reac- 

 tion. Arch. path. Anat. u 

 Phys., Ixxxi, i-6. 

 Kyber denies the advantages of the 

 anilins as reagents for amyloid sub- 

 stance. He admits they are pretty 

 dyes, but for the demonstration of 

 amyloid far inferior to \ irchow's re- 

 action with iodine and sulphuric acid. 



106. Loomis. A simple and speedy 



method of staining animal and 



vegetable sections. Amer. 



Monthly Micr., Journ., i. 143. 



Anilin red. 1-300 is used, and 



cleared in potassium acetate 2-1 of 



water. The preparations bleach out 



in a short time. 



107. Pfitzer. Die Epidermis der 



Amphibien. IMorph. Jahrb.. 



vi, 479. 

 Satranin is recommended as the 

 \ery best nucleus dye. Chromic acid 

 preparations are the best, and after 

 them those made with picric acid. 

 The sections are first washed, then 

 put for a few minutes in a solution of 

 safranin i, absolute alcohol 100, water 

 300 parts, then into absolute alcohol. 

 The color is permanent in dammar, 

 but bleaches in glycerin and water. 



108. Wolff. Zur Bacterienlehre bei 



accidentellen Wundkrankhei- 

 ten. Arch. path. Anat. u. 

 Phys., Ixxxi, 139. 

 Warning is given against certain 

 mistakes liable to occur from the pro- 

 duction of fine precipitates that may- 

 result from the use of anilin dyes on 

 micro-organisms through alkaline re- 

 action of such fluids, as blood. Treat- 

 ment with a little acid by dissolving 

 the precipitate will prevent eri'ors. 



109. Brandt, K. Farbung lebender 



einzelliger Organismen. Biol. 

 Centralbl., iSSi, pp. 202-5. 

 Bismarck brown with hematoxylin 

 is used to stain amoebas. heliozoa. 



flagellates, etc. The stain sliotild be 

 dissolved in the water in which the 

 j organism lives,' i pt. to 3-5000 of 

 fluid. The Bismarck brown stains the 

 oil granifles and the cellulose-like gum- 

 my substance peculiar to the protozoa, 

 and leaves uncolored the nucleus and 

 protoplasm that stain so \ i\'idh' in 

 dead matter. 



1 10. Certes. Sur un procede de 



coloration des infusoires et des 

 . elements anatomiques pendant 



la vie. Zool. Anz., 1881, jjp. 



208-212. Comptes Rendus. 



xcii, pp. 424-26. 

 Ditto. Dosage de la solution de 



Cyanin pour la coloration des 



infusoires. Zool. Anz.. 1881, 



jDp. 287, 288. 

 Like Brand, Certes wished to stain 

 unicellular oi-ganisms. He used 

 cyanin or bleu de quinolein in very 

 dilute solution, i-iooooo, 1-500000. 

 For staining infusoria ordinary water, 

 not distilled, was employed, for 

 white blood and lymph corpuscles, 

 serum. The solutions should be kept 

 in the dark. The oil globules only 

 take this stain, while the nuclei, pro- 

 toplasm, cilia, cuticle and vacuoles 

 remain uncolored. 



111. Flemming. Ueber das Her- 



mannsche Kernfarbungsver- 

 fahren. Arch. Mikr. Anat., 

 xix, pp. 317-330. 

 Ditto. Notiz zur Geschichte der 

 anilin farbungen I.e., pp. 742. 



743-. 

 On trying Hermann's methods 

 (No. 80), using a variety of anilins, 

 it was found a lai"ge numlDcr were not 

 suitable, while some gave very satisfac- 

 tory results. In the first class were 

 eosin, ponceau, and orange, which 

 did not stain the nucleus distinctly. 

 The same deficiency was found in 

 mauvein, fluorescent i"ed, and fuchsin. 

 If applied to chromic acid prepara- 

 tions, even Bismarck brown is not 

 desirable. But the following are very 

 useful and suitable for chromic acid 

 preparations without hardening in 

 alcohol, viz., magdala red, dahlia 

 and especially safranin. Solid green 



