1885.] 



MICKOSCOPICAL JOURNAL. 



215 



This must be carefully observed not 

 to miss the best moment. Lay again 

 in fresh distilled water several times 

 changed. If successful, the gray 

 matter will be more transparent than 

 the white, and the whole section red- 

 dish. If too pale, put again in the 

 dye, if not distinct, soak in the alka- 

 line alcohol. If satisfactor}^ dehy- 

 drate in salt alcohol and mount in 

 balsam. Such preparations are supe- 

 rior to all others in clearness of detail. 

 A part of the gra^• matter onlv is 

 thoroughlv stained called by Weigert 

 • crvthrophile ' or rcdloving, but as this 

 matter surrounds the finest hbrilloc in 

 thin layers the}' become very clear. 



(This method is undoubtedly of 

 great value in the examination of 

 nerves, especially in following the 

 very fine fibrillos, but we think it 

 should be used in connection with 

 the sometimes despised carmine 

 staining. The nerve cells are not 

 colored, and the many details are a 

 serious objection. Seven changes of 

 fluid are required before the section 

 finds a rest in its balsam ; and it is 

 difficult to treat a series of sections at 

 the same time). 



117. Weigert. Ueber Schnellhiirtung 



der nei"\'osen Centralorgane 



zum ZAvecke der Saurcfiich- 



sinfarbung. Centralbl. f. d. 



med. Wiss., 1883. p. 819. 



In order to prepare the material 



for the dye treat it with Miiller's 



fluid or diy in an oven at 30°-40° C. 



about 4 days ; without heat, 8-13 



days is required. Or a fluid of 2^ 



per cent, potassium bichromate and ^ 



per cent, cupric sulphate maybe used. 



118. Ma}er S. Beitrag zur histo- 



logischen Technik. Sitzb. d. 



Wien. Acad.. Ixxxv. Abth., 

 iii, Februar. 

 Violet ' B ' of Bindschedler and 

 Busch is tried for the first tnne, and 

 washed in ^ per cent, salt solution 

 I to 30. Staining requires from a 

 second to i minute. The finer ves- 

 sels are clearly made out, also fatty 

 tissue, the substance and the nucleus 

 of the connective tissue cells. Elas- 



tic fibres appear ultramarine blue on 

 violet ground. Unstriped muscle. 

 and smaller nerves stand out clearly. 

 They must be preserved in potassium 

 acetate, or dried and put in dammar. 

 (It is very difficult to buy a good 

 sample of this dye, and we think it 

 inferior to others) . 



119. Bizzozero. Ueber einen neuen 



Formbestandtheil des Blutes 

 und dessen Rolle bei der 

 Thrombose und Blutgerin- 

 nung. Arch. path. Anat. u 

 Phys. 

 Blood disks are stained with methyl 

 violet, I part concentrated solution 

 in water to 5000, 75 per cent, solu- 

 tion of salt. Uses also gentian violet 

 1-3000 per cent. 



120. Eloui. Recherches histologiques 



sur le tissu connectiv de la 

 cornee, Paris, 1881. 

 Eosin is dissolved in pure glycerin, 

 and fixed by adding alum to the 

 glycerin to saturation. 



(Alum is an excellent mordant 

 for many anilins) . 



131. Errera. La nigrosine comme 

 reactif colorant pour les noy- 

 aux. Pvoces verb. Soc. Beige 

 de micr., i88i,p. 134. 

 Nigrosin soluble in water is rec- 

 ommended as good for nuclei. Per- 

 manent in glycerin and resin. 

 123. Le Vert de Jade. Nouveau 

 reactif colorant. Journ. de 

 Microgr., vi, p. 470. 

 Recommends iodine green. (See 

 no, etc.) I 



123. Strasburger. Ueber den Thei- 

 lungsvorgang der Zellkerne, 

 und das Verhaltuiss der Kern- 

 theilung z u r Zelltheilung. 

 Arch. mikr. Anat., xxi, 

 p. 476. Zellbildung und 

 Zelltheilung 3, Auf., p. 141. 

 A little methyl green is dissolved in 

 I per cent, acetic acid to arrest and 

 fix the figures of dividing cells. 

 These take a temporary color. A 

 solution in dilute glycerin is used to 

 tinge preparations in alcohol, which 

 are fixed in a 50 per cent, solution 

 nitric acid. Staining takes place 



