ANTHRAX 



I8I 



pig, which had died of anthrax. House-flies placed on anthracic 

 meat, seized in the market, also became infected. Graham-Smith 

 (1910, p. 29) carried out experiments with non-spore-bearing 

 anthrax bacilli in blood and with spore-bearing cultures. 



(a) Experiment zvith B. antJiracis in blood (Non-spore- 

 bearing). 



"Twenty-four flies were placed in a cage for one hour together with the body of a 

 mouse just dead of anthrax. The latter had been opened so that the flies could feed 

 on the blood. The flies were then transferred to a clean cage. The next morning the 

 cage contained numerous red spots of vomit, and several masses of yellowish fceces. 

 In the former B. anthracis was found both microscopically and by cultures. The 

 flies were transferred daily to fresh cages and fed on syrup. At intervals specimens 

 were caught and dissected and cultures made, on agar, from their legs, wings, heads, 

 and crop and intestinal contents. Cultures were also made from the fiecal deposits." 



TABLE 26. Showing results of experiments ivith 

 B. anthracis (Non-spore-bearing). 



Cultures from 



Time after 

 infection 



18 hours 



24 



48 



No. of 

 fly 



Legs 



'- 



3 4 



Wings Crop Intestine 



^ I 2 Head M. C. M. C. 



+ + 



- + + 



- - + 



+ + 



3 days 



+ + - 



Crop full of 

 apparently 

 coagulated 

 blood. 



iS 

 '9 



- - - o 



From crop and intestinal contents both Microscopic preparations (M) and Cultures 

 (C) were made. 



