l82 ANTHRAX 



" This experiment shows that non-spore-bearing anthrax 

 bacilli do not remain alive on the external parts of flies for more 

 than 24 hours. They may, however, remain alive in the intestine 

 for three days, and in the crop for five days, especially when 

 partially coagulated blood remains in that organ. Film prepara- 

 tions made at various times from the crop and intestinal contents 

 showed no spore-bearing forms. Plate XVIII, fig. 3, is a re- 

 production of a photograph of a smear made from the crop of 

 a fly (No. 11). The bacilli are present in the faeces deposited 

 48 hours after infection." 



TABLE 27. Shoiving the results of experiments zvitJi 

 anthrax spores. 



Cultures from 



Legs Wings 



Time after No. of ^ '■ ^, , — * ^ Intes- 



infection fly 12345612 Head Crop tine Fseces Vomit 



- ti 



hours... I 000000 ^-o+++++ + 



2 + + + + + + + + ++ f + ++ o + 



3 o + + + + + + + ++ ++ ++ +(3 out of 4) +(^soutof5) 



4 + + + + + + + +■+ 4- + + 



5 + + + + + + + + + ' - ++ +(3 out of 4) 



6 + + + + + + + + + - ++ +(8 out of 8) +(5 out of 5) 

 ( 7 + + + + + + + ++ -+ + 

 '( 8 + + + + 0000++ - + + 



9 + + + + + + + + + ++ + + 



i 10 +00000 + + ++++. + (i out of 5) +(i out of 4) 



3 days -, 11 + + + + 00 + + +++ + 



I 12 + + + +00 + ++ - + + 



f 13 + + o o o - + + + + ++ +(3 out of 5) +(4 out of 5) 



I 14 +00000 + ++ ++ + 



f 15 00000000 o +++++(3 out of 7) o out of 3 



( 16 00000000 o ++ + 



17 + + 000000 + ++ + o out of 4 +(ioutof 5) 



... 18 00000000 o ++ ++ o 



16 



19 + + 000000 + o 



20 00000000 o o o +(i out of 7) 



21 +00000 + 00 - +0 



22 00000000+ O 00 



23 000000000 o 00 



24 000000000 o o- 



25 00000000 o o o 



26 000000000 + o- 



" Cultures were also made from drops of .syrup after the flies had been allowed to 

 feed on them. Anthrax bacilli were cultivated on the 3rd, 4th, 6th, 7th, 8th, and 

 loth days, but not later." 



