Hill J Notes on the Colorado Potato Beetle 81 



purpose of rearing a sufficient quantity of the second generation, 

 six lots or batches of eggs numbered from a to/. In all 49 adults 

 were obtained from the six batches. For clearness, the batches 

 are treated in detail: (1) Batch a, consisting of about 20 

 (number unknown) eggs hatched at 6:30 A. M., July 7, the 

 resulting larvae entering the soil for pupation at the average 

 time of 7 a. m., July 19 and on July 30 and 31, 4 adults 

 were obtained. These were at once fed and at 3 P. M., August 

 3, a pair were observed mating and were isolated. This pair 

 continued to mate until 9 A. M., August 7, the o entering the 

 soil shortly afterward; with them mating was observed five 

 times, but no oviposition occurred. In the meantime, the 

 two remaining beetles had hibernated (August 8), the mated 

 female following a week later. Hibernation induced by star- 

 vation due to lack of time in which to feed the beetles. (2) 

 Batch b, consisting of 00 eggs came to larval maturity at 8 A. M., 

 July 24, and on August 4 and 5, two adults were obtained 

 comprising the whole survival. These were males and hiber- 

 nated on August 22. (3) Batch c, 58 eggs, came to larval 

 maturity at 9 P. M., July 25, and gave from August 5 to 

 7, 4 adults which were placed on food as they emerged. A 

 male died on August 8 and a pair were mating at 4 P. M., 

 August 9; this pair was then isolated. On August 11 at 

 10 a. M., 3 eggs were deposited which proved to be fertile; 

 mating was again observed at 3 P. M. the same day and at the 

 same hour on August 12; later the same day (12) 9 eggs 

 were deposited on a leaf, which also proved to be fertile; another 

 mating was observed at 9 A. M., August 13, but thereafter no 

 other matings were observed and further reproduction did not 

 occur. The remaining adult died on August 22, but the 

 mated pair remained alive without food until August 25, 

 when the cage was broken up. Oviposition and mating in 

 spite of insufficient food. (4) Batch d, 43 eggs, came to larval 

 maturity at 10 P. M., July 24, and on August 4 from 7 to 

 10 A. M., 11 adults were obtained, the total survival. On 

 August 9, 3 pairs observed mating were isolated but other 

 matings did not occur with them nor oviposition, caused as we 

 have reason to believe, by the neglect to supply food. Thus, 

 on August 13 the third pair had entered the soil for hibernation 

 and two days later the second pair had done likewise; 

 the first pair remained on top of the soil until August 25, 



