﻿PROCEEDITSTGS 
  OF 
  THE 
  FOURTH 
  ENTOMOLOGICAL 
  MEETING 
  257 
  

  

  emergence 
  of 
  lac 
  larvae 
  so 
  as 
  to 
  facilitate 
  cutting 
  and 
  transportation 
  

   of 
  broodlac 
  long 
  distances 
  either 
  by 
  rail, 
  post 
  or 
  steamer. 
  

  

  The 
  method 
  advocated 
  herein 
  is 
  not 
  final, 
  and 
  it 
  is 
  possible 
  that 
  other 
  

   workers 
  in 
  the 
  field 
  would 
  be 
  able 
  to 
  suggest 
  some 
  other 
  more 
  simple 
  

   method 
  than 
  the 
  one 
  detailed 
  here. 
  But 
  for 
  the 
  time 
  being, 
  an 
  exami- 
  

   nation 
  of 
  the 
  ovaries 
  would 
  give 
  sufficient 
  data 
  wherewith 
  to 
  know 
  the 
  

   probable 
  date 
  of 
  the 
  emergeni3e 
  of 
  larvae. 
  I 
  have 
  tried 
  this 
  method 
  

   personally 
  for 
  some 
  years 
  past, 
  and 
  find 
  it 
  gives 
  sufficiently 
  accurate 
  data 
  

   wherewith 
  to 
  fix 
  the 
  emergence 
  of 
  the 
  larvae 
  in 
  distant 
  parts 
  of 
  the 
  

   country. 
  In 
  the 
  past 
  I 
  have 
  examined 
  broodlac 
  for 
  the 
  emergence 
  of 
  

   larvae 
  from 
  Sind, 
  Baroda, 
  Assam, 
  Banganapalle, 
  Karauli 
  and 
  Rewah, 
  

   and 
  found 
  this 
  method 
  sufficiently 
  indicative 
  of 
  fixing 
  the 
  dates 
  of 
  

   emergence 
  with 
  greater 
  degree 
  of 
  certainty 
  than 
  would 
  have 
  been 
  the 
  

   case 
  otherwise. 
  

  

  The 
  accessories 
  required 
  for 
  this 
  work 
  are 
  : 
  — 
  

  

  A 
  compound 
  miscroscope. 
  

  

  A 
  dissecting 
  microscope 
  or 
  a 
  Greenough's 
  Binocular. 
  

  

  A 
  few 
  clean 
  slides. 
  

  

  A 
  scalpel. 
  

  

  Two 
  or 
  three 
  pipettes 
  (one 
  medium, 
  one 
  fine). 
  

  

  Three 
  or 
  four 
  stout 
  mounted 
  needles. 
  

  

  Three 
  or 
  four 
  watch 
  glasses. 
  

  

  Distilled 
  water. 
  

  

  The 
  only 
  difficulty 
  that 
  is 
  likely 
  to 
  be 
  experienced 
  by 
  most 
  of 
  the 
  

   growers 
  is 
  that 
  they 
  cannot 
  provide 
  themselves 
  with 
  the 
  equipment 
  

   given 
  above. 
  The 
  microscope 
  and 
  the 
  dissecting 
  microscope 
  or 
  the 
  

   binocular 
  require 
  a 
  very 
  heavy 
  outlay, 
  far 
  beyond 
  the 
  reach 
  of 
  an 
  

   ordinary 
  grower. 
  But 
  the 
  method 
  advocated 
  above 
  is 
  more 
  or 
  less 
  

   a 
  laboratory 
  method 
  intended 
  for 
  those 
  who 
  have 
  some 
  knowledge 
  of 
  

   microscopic 
  technique 
  or 
  are 
  working 
  in 
  a 
  laboratory 
  fitted 
  with 
  the 
  

   above 
  things. 
  All 
  that 
  is 
  required 
  is 
  to 
  take 
  a 
  few 
  cells 
  on 
  a 
  stick, 
  

   remove 
  the 
  resin 
  round 
  the 
  insects 
  either 
  with 
  a 
  scalpel 
  or 
  a 
  teaser, 
  

   make 
  a 
  longitudinal 
  incision 
  with 
  a 
  pointed 
  needle, 
  and 
  take 
  out 
  a 
  

   portion 
  of 
  the 
  ovaries, 
  on 
  a 
  clean 
  slide. 
  The 
  specimens 
  are 
  then 
  washed 
  . 
  

   either 
  in 
  normal 
  saline 
  solution 
  or 
  in 
  distilled 
  water, 
  adjusted 
  under 
  

   the 
  binocular 
  and 
  examined 
  under 
  the 
  microscope, 
  A 
  comparison 
  is 
  

   then 
  made 
  with 
  the 
  figures 
  in 
  the 
  accompanying 
  plate, 
  and 
  the 
  period 
  

   determined 
  for 
  the 
  particular 
  locality 
  from 
  which 
  the 
  specimens 
  have 
  

   either 
  been 
  obtained 
  or 
  collected. 
  

  

  In 
  Mysore 
  A. 
  falcateUa 
  is 
  very 
  rare, 
  Euhlemma 
  is 
  our 
  worst 
  pest, 
  Mr. 
  Subiramaniam. 
  

   There 
  is 
  no 
  systematic 
  cultivation 
  of 
  lac 
  in 
  Mysore, 
  

  

  