1899] MICROSCOPICAL JOURNAL. 75 



At the apex of the teleutospore there is a single per- 

 foration only, or at any rate a thinning of the outer cell- 

 wall which looks like it, and it is through this that the 

 germinating process protrudes ; in other words, the exo- 

 spore is pierced by the endospore in germination. When 

 the teleutospores are bicellular the germ-pore of the lower 

 cell occurs just below the median septum. The germ 

 tube of the teleutospore does not form a mycelium, but is 

 broken up near the apex into three or four short cells, due 

 to the formation of transverse septa. Each cell gives or- 

 igin to a very slender outgrowth or sterigma, the apex of 

 which eventually bears a pi'o-mycelial spore, capable of 

 producing a mycelium. 



Practical Hints on Mounting. 



R. G. MASON. 



Farrant's Medium. — This is another preparation of 

 glycerine and gum. It is useful for some anatomical prep- 

 arations, Epithelium, etc. Upon a clean slip, place a little 

 of the media. This must be carefully done as this mix- 

 ture holds air bubbles with greater tenacity than any- 

 thing I know of. It is best to warm the slip first over a 

 lamp. Now having the object in soak in a weak solution 

 of the above media and water, place the object in position, 

 warm a cover glass, and cover gently, press down, set 

 aside to dry. If, on examination, air is imprisoned un- 

 der the cover with the specimen, the whole process must 

 be repeated. The bubbles will not disappear by them- 

 selves as in the. case of balsam and benzole. This media 

 will take some days to dry. So it is best to look at the 

 slides every day and press down if required. When fair- 

 ly hard at the edges, clean off with a soft brush and water, 

 set aside to dry, run a ring of cement round as before, 

 and finish in the usual way. 



Likely Failures, — Air bubbles round object or in media. 



