1899] MICROSCOPICAL JOURNAL. 179 



1. Add about 30 drops of the solution of hoematoxylin 

 to an ounce of distilled water, and stain the section 

 for fifteen to twenty minutes. 



2. Wash well in distilled water. 



3. Soak for a few minutes in ordinary tap water until 

 the color becomes blue. 



4. Place in strong methylated spirit and dehydrate 

 for at least ten minutes. 



5. Place in clove oil to clear for about five or ten min- 

 utes. 



6. Mount in Canada balsam. 



Most stems, roots and leaves can be double stained with 

 the following staining solutions : 



BORAX CARMINE. 



Pure carmine 1 dram. 



Liq. ammonia 2 drams. 



Dissolve the ammonia and add twelve ounces of satura- 

 ted solution of borax in distilled water; filter and keep 

 in a stoppered bottle. 



1. Place the section in a little of the above stain in a 

 watch glass for about five minutes. 



2. Wash well in methylated spirit. 



3. Take of hydrochloric acid, 1 part ; and of methy- 

 lated spirit, 20 parts. Mix together and soak the sec- 

 tion until it becomes of a bright scarlet color ; if 

 over stained, until the excess of stain is removed. 



4. Wash well in methylated spirit. 



5. Make up an alcoholic solution of Grrubler's acid ani- 

 line green in methylated spirit, about two grains to 

 an ounce of spirit, filter and immerse the section for 

 ten to fifteen minutes. 



6. Wash well in methylated spirit; if over stained 

 with the green, soak until the excess of color is re- 

 moved. The different parts of the specimen should 

 be distinctly visible to the naked eye^-woody tissues, 

 green ; parenchyma, red. 



