1895.] MICROSCOPICAL JOURNAL. 199 



the dorsal, one from the lumbar. Let the cut surfaces 

 be perpendicular to the long axis of the cord. Place 

 them in 300 or 400 grms. of Erlicki's fluid, made thus: — 

 Bichromate of potash, 2 grms. ; sulphate of copper, 

 0.50 ; water, 100. At ordinary temperature, hardening 

 will be effected in from 10 to 12 days ; in an oven at 37° 

 C, in 4 or 5 days. It is well to renew the fixatine once 

 or twice. Wash in water for 24 hours, then place in 

 alcohol. 



Stainmg ; imbedding. — Imbed a piece a half a centi- 

 metre long in celloidin. Place the sections in alcohol ; 

 stain by Wiegert's method ; mount in balsam. 



Medulla oblongata ; cerebrum ; cerebellum. — Fix, stain 

 and imbed as already described for the cord. The con- 

 volutions of the cerebrum and of the cerebellum furnish 

 the following preparations : — (1) Dissociate the gangli- 

 onic cells after maceration in the I alcohol. (2) Section 

 after hardening in Erlicki's fluid. Stain in the alcoholic 

 boracic carmine, or by Weigert's method ; imbed in cell- 

 oidin ; mount in balsam. 



DIGESTIVE APPARATUS. 



Mucous mernbrane of the mouth and lips., — Section, 

 after fixing by alcohol and hardening by alcohol and 

 gum. Stain in picro-carmine, cover, replace the staining 

 fluid by glycerine. 



The lamellar cells formitig the most superficial layer 

 of the buccal epithelium may be easily isolated. Scrape 

 the inside of the cheek with the finger nail, spread the 

 cells on a slide in a drop of saliva, expose to osmic acid 

 vapor, stain in picro-carmine. 



Mucous membrane of the tongue. — Beautiful prepara- 

 tions may be made by fixing and hardening pieces in 

 alcohol and gum. Section, stain in picro-carmine, 

 mount in neutral glycerine or with a little picro-car- 

 mine added. 



