1895] MICROSCOPICAL JOURNAL. 201 



Fix another piece by ammonia bichromate, 2 per cent, 

 for 8 days ; wash several times thoroughly ; harden in 

 gum and alcohol, section, stain in hsematoxylin and 

 eosine, and mount in balsam. 



The rabbit's oesophagus affords excellent material for 

 studying the nerve endings in the muscle. Place a lig- 

 ature at the lower part, fill the tube with fresh lemon 

 juice. In a few minutes wash lightly and replace the 

 lemon juice by a 1 percent chloride of gold solution ; tie, 

 to keep the tube slightly distended; cut out the part be- 

 yond the two ligatures and place for 20 minutes in a 

 bath of gold chloride. Slit the segment of oesophagus, 

 put it for 24 hours in the i acid. It is very easy to 

 strip off a shred of the muscular layer and to mount it 

 flat in glycerine. 



The Stomach. — In that of the frog, study the epithel- 

 ium lining the gastric mucous membrane. Open the 

 stomach and macerate it in the I alcohol. In 24 hours, 

 scrape the inner surface with a scalpel, spread the pro- 

 duct on a slide in a drop of the I alcohol , add picro-car- 

 mine and mount in glycerine by the partial desiccation 

 method. 



Sections. — Remove the stomach with an end of the 

 oesophagus and two or three centimetres of the small 

 intestine attached. Dilate the stomach by injecting 

 through the oesophagus the fixing fluid, after tying the 

 small intestine. Tie the oesophagus and put the whole 

 in a bottle of the fixing fluid. Absolute alcohol or os- 

 mic acid are useful as fixatives. Section after 1 hour in 

 absolute alcohol. If osmic acid be used, let it act for 

 half an hour, wash for one or two hours, harden by 

 gum and alcohol, section, stain by alum carmine, pre- 

 serve in glycerine. 



Stomach of a mammal. — Select that of a dog, of a 

 cat or of a rabbit. Remove a portion from the cardiac 



