THE AMERICAN 



MONTHLY 



MICROSCOPICAL JOURNAL. 



Vol. XVI. OCTOBER, 1895. No. 10. 



A Special Apparatus for Bacteriological Sampling of 

 Well Waters. 



WITH FRONTISPIECE. 



Bv H. Tv. BOIvLEY AND MERTON FIELD, 

 N. DAKOTA. 



Read lieCorethe Botauical Club of the A. A. A. S., at Spriiigfielfl raeetiug, 

 August 30, 1895. 



To those engaged Id bacteriological analysis of waters, 

 proper sampling of the same commends itself as a ne- 

 cessity to the attainment of definite results. To be of 

 use, this must be accomplished so as to take the desired 

 amount from the point or depth in the body of water 

 wished. 



Different workers have used various methods and dif- 

 ferent types' of apparatus. These sampling pieces have 

 usually been vacuum tubes capable of being sealed by 

 flame or else glass-stoppered bottles carefully sterilized. 

 These do not seem satisfactory either as to rapidity of 

 work allowed or for accuracy. For example, the small 

 vacuum tubes of the Pasteur pattern are difficult to fill 

 at any great depth of water under bacteriological condi- 

 tions, and equally difficult to seal in the flame so as to 

 prevent air contamination. Lepsius' self-filling, deep 

 water sampling apparatus (see Sternberg's Manual, p. 

 555) is out of the question, as being entirely too cumber- 

 some and because of the difficulty of sterilizing the mer- 



