1889.] MICROSCOPICAL JOURNAL. 31 



Connective tissue stains yellow ; epidermis, the rete Malpighi and 

 the analogous layer in the mucous tissue, muscle, and labial glands 

 stain light red, die nuclei standing out sharply. 



Metanil Yellow and Crystal Ponceau [Alphaazonaphthallin- 

 disulfobetanaphthol of soda]. — Preparation: spinal cord of calf, alco- 

 hol hardening. 



For the single as well as the combined stain, twenty-four hours are 

 required. 



The gray matter stains yellow, the white reddish. Under strong 

 magnification the neurolgia and connective tissue are found to be stained 

 yellow ; the axis cylinders dark bluish red ; the myelin light yellowish 

 red ; one sort of ganglion cells dark purple, another bluish red ; nuclei 

 do not stand out sharp. 



Metanil Yellow and Congo Red [Tetraazodiphenyldinaphthyla- 

 mindisulphonate of soda]. — Preparation: spinal cord of calf, alcohol 

 hardening. 



The sections are stained in a mixture of the staining fluids for 8 min- 

 utes, or they are stained for io minutes in the yellow stain and then for 

 5 minutes in the red. 



Ganglion cells [without clear nuclei staining] and axis cylinders stain 

 dark violet-red ; the medullary sheath light citron-yellow ; neurolgia 

 and all connective tissue light violet-red ; epithelium of the central canal 

 brownish -red. 



Carminate of Soda and Metanil Yellow. — The central nervous 

 system is hardened in Midler's fluid, then stained in toto with the car- 

 mine fluid. Sections are then stained for ten minutes in the yellow 

 stain. 



All nervous elements are stained red, all connective tissue elements 

 yellow. 



Crystal Poneeau and Crystal Violet. — Preparation : transverse 

 section of the carotid of the calf, alcohol hardening. 



The sections are stained for 5 minutes in the red ponceau fluid and 

 then for 1 minute in the violet. 



Nuclei of the endothelium and smooth muscle stain violet ; all the 

 other tissues' red. 



Congo Red and Anisol Red [Bisulfoxylnatronbetaoxynapthalina- 

 zoorthometoxylbenzol]. — Preparation : spinal cord of the calf, alcohol 

 hardening. 



The sections are stained for 5 minutes in the combined stains, or for 

 5 minutes in the Congo red solution and then for 5 minutes in the anisol 

 red. 



Axis cylinders and cell bodies stain purple ; all other tissues stain 

 light red. Nuclei do not stain. 



Metanil Yellow and Athylin Blue. — Preparation : ala nasi of a 

 child, alcohol hardening. N 



When the two staining' solutions are combined a black precipitate is 

 formed, which re-dissolves in an excess of the metanil yellow solution. 

 This solution stains yellowish-green, the cartilage only being stained 

 blue If the sections are first stained for 5 minutes in a mixture of 5 

 c.c. of the yellow and 4 c.c. of the blue stains, or if the sections are 

 stained for ten minutes in the yellow and then for 2 minutes in the blue, 

 the pictures will be shai-p. 



