LEVINE: CyTOLOGY oF HYMENOMYCETES 157 
Compare the astral rays in FIG. 72, 73, PL. 6 and FIG. 51, 53, PL. 8, 
with those shown in FIG. 24, 62, PL. 7 and FIG. 58, PL. 8. Well- 
developed polar asters are shown in my preparations of Boletus 
castaneus, B. glavellus, B. vermiculosus, B. versipellis, B. chrys- 
enteron, B. punctipes, B. griseus, B. subtomentosus, and B. cyanes- 
cens. 
The number of chromosomes is difficult to determine but in 
favorable sections I have been able to count from six to eight. 
They lie as shown by Wager (1894), Ruhland (1901), Juel (1897), 
Harper (1902) and others in the center of the spindle although no 
characteristically dense equatorial plate is formed (PL. 6, FIG. 72, 
73)- 
The chromosomes are drawn to the poles and at the same 
time the spindle elongates until its ends (PL. 6, FIG. 25; PL. 8, 
FIG. 54, 55) touch the basidium wall. Astral rays may be still 
seen radiating in all directions from the point of contact. The 
chromosomes become densely aggregated at the poles. I have 
been able, however, to see distinctly, in perfect fixations, as many 
as five chromosomes just before they reached the poles (PL. 6, 
FIG. 25a). Undoubtedly the appearance of two chromosomes in 
the equatorial plate and diaster stages, as reported by Maire (1902) 
and Van Bambeke (1903), indicates fusion of the chromosomes due 
to imperfect fixations. 
In the reconstruction of the daughter nuclei, a nuclear mem- 
brane is formed about the chromosomes. The latter begin to 
stream out (PL. 6, FIG. 26) forming a reticulated structure, and small 
nucleoles appear. The resulting nuclei resemble in all respects 
the mother nuclei. In FIG. 56, PL. 8, the two daughter nuclei are 
shown attached to the basidium wall. Faint astral rays are still 
visible coming from the point of contact. The old nucleole may be 
seen lying in the cytoplasm. This persistence of the kinoplasmic 
rays is conspicuous, but they disappear before the second spindle 
is formed. The two daughter nuclei prepare immediately for 
the second division. The prophases are hard to study. I have 
observed in Boletus versipellis, that the nucleoles come to lie on 
the side of the nucleus toward the base of the basidium (PL. 8, 
FIG. 57) and pass out into the cytoplasm, after the disintegration 
of the nuclear membrane. The spindles show centrosomes with 
