172 LEVINE: CyToLOGyY oF HYMENOMYCETES 
somes in the second division is certainly greater than two. The 
number which can be distinguished is variable but more than two 
are always found. This is true even in the case of the very small 
karyokinetic figures of the second division in the spore as shown 
in FIG. 68, PL.8. That we havea reduction division in the basidium 
comparable to that found in the spore mother cells of the higher 
plants is hardly to be questioned on general grounds; but the 
diminutive size of the chromosomes makes the study of the 
process extremely difficult. It is certainly clear, however, that 
Maire’s (1902) and Van Bambeke’s (1903) two chromosomes in | 
both divisions, and Fries’ (1911) two chromosomes in the second 
division and in the division in the spores are the results of poor 
fixation causing the fusion of the chromosomes. 
My observations on the Boleti confirm the views of Wager 
(1903, 1904), Juel (1897), and Harper (1902), and it cannot be 
doubted that Maire’s protochromosomes are the true chromo- 
somes. | 
Maire (1902) and more recently Fries (1911) have given 
extremely interesting data as to the migration of the four nuclei 
from the basidium into the spore. The nuclei according to their 
observations are drawn into the sterigmata by fibrillar cyto- 
plasmic strands which extend from the sterigmata to the nuclei. 
My preparations show clearly also the kinoplasmic strands extend- 
ing from the nuclei to the points of origin of the sterigmata (PL. 7; 
FIG. 36; PL. 5, FIG. 37). Petri held that these strands originate by 
stretching of the nuclear membrane. The daughter nuclei in the 
telophases become attached to the basidium and when they move ~ 
downward in their well-known migration, the nuclear membrane is 
drawn out into a long fibrillar strand. The strand may be com> — 
pared toa much attenuated beak on the nucleus. Another interpre 2 
tation of these strands is possible. As noted in the telophases ° 
the second division, the daughter nuclei come to lie on the wall of 
the basidium and apparently their positions mark the point of . 
origin for the future sterigmata. When the nuclei migrate dow? 
ward in the basidium, the centrosomes are left behind on t 
basidium wall. The sterigma, budding out at just this point 
carries the centrosomes upward (PL. 5, FIG. 37), at its apex. The 
strands connecting the centrosomes and nuclei are thus carti 
