Hoyt: CULTURES OF SPIROGYRA 363 
TABLE I 
CULTURES OF SPIROGYRA IN NUTRIENT MEDIA PREPARED WITH DISTILLED WATER 
Nature of culture medium 
No. of | Author and concen-| Kind of dis- | Agar added! Duration of cul- Condition of 
culture | tration, per cent of | tilled water | to medium, tures, days plants * 
? total salts in used in me- | fer cent by 
medium dium weight 
I Sachs, 0.05 — 3 
2 0.05 nontoxic —_ 4 E 
3 0.10 ordinary 10, 20 Pd, Pd 
4 0.10 oO. 1.0 34 P 
5 0.10 nontoxic 40, 18, 44 E, Eg, Gp 
6 0.50 ordinary — 3,14 D,D 
7 0.50 oO. 1.0 60 E 
8 0.50 nontoxic — 50 G 
9 1.00 ordi — 2 D 
Io I.00 nontoxic — 44 P 
II Knop, 0.05 ordinary oe 3:3 D,D 
12 0.05 nontoxic — 48 Gp 
13 0.10 di — 3 D 
14 0.10 do. 1.0 49 Pd 
15 0.10 pence a 18, 31, 44,11 | G,t Pd, Pd, Pd 
16 0.50 i a 18, 49, I G,t is D 
17 0.50 salen “= 23;.2 Pd, D 
18 1.00 ordinary — Pd 
19 1.00 | nontoxic —< I D 
20 | Molisch, 0.05 ordinary — 3 D 
2r 0.05 nontoxic — 48 E 
22 0.10 | ~ ordinary ee 7 Pd 
23 0.10 oO. 1.0 49 - 
24 0.10 nontoxic 62, 62, 40,40 | E,G,G,G 
25 0.50 ordinary 555 48, 22, 14 G, Pk. Pd 
26 0.50 do. 1.0 66 E 
27 0.50 nontoxic sae 50 Eg 
28 T.00 ordinary —_— 46 P 
29 T.00 nontoxic eae: 51 P 
30 Crone, 0.05 ordinary = 3 D 
31 0.05 nontoxic — 48 E 
32 0.10 ordinary 3 D 
33 0.10 ; 1.0 8 Pd 
34 0.10 | nontoxic 23, 40, 40,51 | E, E, E.G 
35 0.50 ordinary aa 48, 13, 14 Eg,t Pd, Pd 
36 0.50 do. 1.0 E 
37 0.50 nontoxic — 50 2 
38 I.00 ordi ca 82 G 
39 1.00 nontoxic 51 Gp 
* The ene nee ob the cont is indicated as follows: E, excellent, G, 7, good; 
P, poor; D, dea cimacoengr ier 
by each of ae letters—as, Eg indicates a condition between excellen , ete, 
the experiment wae i . oO several notations giv en rele ete 
single experiment. The numbers in eceding column (duration in days) refer 
to the experiments in the same order as do the letters; thus, I nico 
condition and the first number denoting duration both refer to the same experimen 
etc. 
+The amount of medium used was unusually small, in comparison with the 
number of filaments in the culture. 
