354 Hoyt: CULTURES OF SPIROGYRA 
TABLE II 
CULTURES OF SPIROGYRA IN TAP WATER, WITH TREATMENTS AND ADDITIONS 
, Duration 
No. of ns of Condition 
culture Treatments of, and\additions to, tap water cultures, of plants* 
days 
2 Ue $4,477 IP, DED 
2 paige gibt on to glass 13,44, 2: (P-Pd,D 
, first portio at, P,D 
3a rote 2, middle portion 3, 12 Gp, Gp 
30 |As 2, final po 12,3 Pop 
oiled to A ae ori E812 Eg, P 
5 vaporated Hi orig vol G 
5a ntreated (control to 5 I Ps 
Evaporated to 1/6 orig. vol I Gp 
6a |Untreated (control I Pd 
if Evaporated to 1/3 orig. vol I D 
7a |Untreated ac to 7) r D 
8 {15 mins. at 1 37 E 
8a Ppa: ‘onteol to 8) 37 Pd 
9 |45m 46 Pd 
10 Distillate ees animal charcoal, glass to glass 1 to 44 |E, E, E, 
Eg, Eg, Eg, 
Eg, G, P 
Ir |Boil ith animal charcoal to 1/8 orig. vol., filtered| 26 
Ila naar from 26 
11b |Do., charcoal rinks Ir added to culture. 26 Gp 
12 |As 11, filtered 16 G 
12a |As ft, not filtered 16 Gp 
12b |As 11a 16 G 
12zc |As11b 16 Gp 
13 |Untreated plants from stock jar} 6 G 
14 |As 13, but plants from Crone’s sol.f 6 E 
* The condition of th 1 as foll E, excellent; G, good; P, poor; 
D, dead. Acombinati of letvecn dent condition between that denoted by each 
of these letters, as: Eg indicates a condition between excellent and good, etc. Where 
the experiment was repeated, each of the several notations given refers to a single 
experiment. The numbers in the preceding column (duration in days) refer to the 
experiments in the same order as do the letters; thus, the first letter denoting con- 
dition and the first number denoting duration both refer to the same experiment, etc. 
Tt Nos. 13 and 14 were simultaneous, in the same dish 
