358 Hoyt: CULTURES OF SPIROGYRA 
CULTURES OF SPIROGYRA IN SALT SOLYTIONS PREPARED FROM NONTOXIC WATER, WITH 
ND WITHOUT ADDITIONS 
Salts in solution, per cent es 
No. of Additians Duration of} Condition of 
culture | ¢ NO; [KeHPO,| MgSO,| KCI Oe TS ee 
ET: | Ost 0.1 0.1 0.1 — 13, 28,8 | Gp, Gp, Pd 
Ia@ | 0.025 | 0.025 | 0.025 | 0.025 — 26, 29 Gp, P 
2 o.1 0.1 0.1 0.1 Animal charcoalf 
2a | 0.025 | 0.025 | 0.025 | 0.02 0. 26 Gp 
Si 0.1 0.1 0.1 Wood charcoal I5 Pd 
3a | 0.025 | 0.025 | 0.025 | 0.025 2 35 G 
4 do., do. do. do. jCaCle 28 G 
. do. do. do. do. |CaCOs, t 35 Eg 
5a ‘ do. do. do. |CaCO; abundance 35 E 
6 0.133 | 0.133 — | 0.13 2 D 
6a | 0.03 0.033 — | 0.033 — 2 D 
7 0.133 | 0.133 | — | 0.133 |Animal charcoalf 3 D 
8 5 do, —_— do. |CaCOsz 6 Be 
9 0.1 0.1 — |o.1 CaCle 0.1 p.c. 5 D 
10 do. do. | 0.1 do. —_ 6 Pd 
II ae — 10.4 — a 3,2 D,D 
Ila _ — 0.1 —_ —_— a2 DD. D 
11b —_ — | 0.05 —_— — 2 D 
Tie _ — | 0.01 —_— 6,2 Pd, D 
12 a — \o4 — jAnimal charcoalt 2 D 
I2a —_ — “| O2% —s do. 2 D 
12b — — | 0.05 _— do. 2 D 
I2¢ — — | 0.01 oo do. 9,5 PoP 
13 — — |04 — |Wood charcoal{ re do: 
13a _ — |o.1 — i Pd 
14 — — |04 — |CaCle 0.1 fa 15 P 
14a ee — | 0.1 — : baa Pd 
15 — do. | 0.1|| 14 P 
16 — — |o4 — |CaCOs ings pater 14 Pd 
17 _ do. — |CaCOs, in I Gp 
18 — — | 0.01 —_— voyensie cl. Pt 90 p.c.| 19 Gp 
18a —_ do. — |Pt Cl 8 p.c. 2 D 
19 — — |0.4 2 D 
20 — _— — |0.1 a 6 Pd 
21 — a — do. |Colloid. sol. Pt 12 - : 
22 — — do. |CaCl: 0.1 p.c. 7,6 Gp,° Pd 
23 — —_ — — jCaCl: 0.2 p.¢ a2 D,AD 
24 — — o.r |CaCle o.t p.¢ 4 G° 
25 — — —_ — |CaCle 0.2 p.c. 7 G4 
* Th d ] E, excellent; G 
t ; P, poo 
D, dead. A combination of letters denotes a condition between. oat ae oe 
indi 
each of these letters—as, Eg cates a condition between excellent and good, etc. 
Wh experiment was , each of t veral ions gi refers to a 
single experiment. The numbers in the preceding (duration in days) refer 
to the Sagundeprninge in the same order as do the letters, ur 2 the — letter denoting 
condition and the first number denoting duration both refer to the same experiment, 
etc. 
+ Culture 23 days old showed n um with acetic acid and ium oxalate 
TA culture 14 days old denied no Rag re 
Containing some calcium. 
|| Showed no calcium. 
° Spirogyra from culture in Beacon’ s solution, 66 days old, was used in 24 and 25- 
The first test of the stock material (22) and of material from Crone’s solution (24) 
bie mage carried out in the same 
he second of the Spirogyra (23) and of material from Crone’s 
stock 
kale (25) were nerranronand carried out in the same 
