IN THE MOTOR ORGANS OF LEAVES. 



17 



completely flaccid and weighed 8*06 gran inn-, equivalent to a loss of 19*6 per cent, on 

 the original weight and to an exudation of I'iCcc. of liquid. 



Experiment XIII. — A leaf of Kalanchoe, weighing 27*93 grammes, was set in a 

 carbonic acid chamber. Visible exudation began within the course of an hour and 

 advanced rapidly, accompanied by collapse of the leaf. The reddish colouring along 

 the margins of the lobes was, primarily at all events, Intensified, and t ho rest of the 

 surface gradually became yellowish. On the following day the leaf was moist, flaccid, 

 and weighed only 24*61 grammes, indicating a loss of 11*8 per rent. 



Experiment XIV.—' A leaf of Kalanchoe, weighing 13*04 grammes, was suspended 

 over water in a carbonic acid chamber. It remained in the chamber for forty-eight 

 hours, and at the close of that period was flaccid, of a yellowish olive colour, and 

 weighed only 11*54 grammes, indicating a loss of 15-3 per cent, of total weight. 



Experiment -XT.— A leaf of Kalanchoe, weighing 33*22 grammes, was set in a 

 hydrochloric acid chamber. Visible exudation and brightening of the red colour at the 

 margins of the lobes soon appeared. On the following day it was flaccid and of a dull 

 yellowish olive colour, and weighed only 28*51 grammes. 



Experiment XVI.— A leaf of Kalanchoe, weighing 18*12 grammes, was set in a 

 hydrochloric acid chamber. Visible exudation was present within a quarter of an hour. 

 Twenty-four hours later it was very moist, quite flaccid, of a yellowish olive colour, and 

 weighed only 15*02 grammes. 



Experiment XVII.— A leaf of Kalanchoe, weighing 14*62 grammes, was placed in 

 a nitric acid chamber. On the following day it was of a yellowish olive colour with 

 the edges of the lobes strong pink. It was not so moist or flaccid as a leaf treated 

 at the same time in a hydrochloric acid chamber, but showed a loss of 15*5 per cent. 

 in weight. 



Experiment XVIII.— A leaf of Kalanchoe, weighing 13*08 grammes, was set in a 

 nitric acid chamber. Visible exudation occurred within two hours, and on the following 

 day it was perfectly flaccid, of a dull olive with pinkish along the margins of the 

 lobes, and weighed only 10*23 grammes, having lost 21*7 per cent, in total weight. 



Experiment XIX.— A leaf of Kalanchoe, weighing 14*13 grammes, was set in an 

 osmic acid chamber. Blackening of the surface rapidly set in, and on the following day 

 it was coal-black and covered by large isolated drops of inky fluid. It was not, 

 however, flaccid, and only showed a loss of 7*2 per cent, in weight, whilst a leaf 

 which had been exposed simultaneously in a nitric acid chamber showed one of 21*7 

 per cent. Twenty-four hours later it was still nearly rigid, but had discharged a 

 considerable additional amount of black liquid and only weighed 12*51 grammes. 





Experiment XX.— A leaf of Kalawhoe, weighing 15*17 grammes, was set in an 

 osmic acid chamber. It began rapidly to blacken at the edges, and at the close of 

 two hours the discolouration had spread inwards considerably, and one or two small 

 drops of black exudation had appeared on the surface of all the lobes save one. There 



Ann. Roy. Bot. Gard. Calcutta Vol. VI 





