18 



THE CAtTSES OF FLUCTUATIONS IN TUEGESCENCE 



was no general moistening of the surface or any indication of commencing flaccidity. 

 Three hours after the beginning of Jhe experiment it was very greatly blackened, but 



was still quite 



rigid 



d had lost 



07 grammes in 



weight 



On the 



following 



morning it was perfectly black, but had discharged very little more fluid, the texture 



remaining 



quite firm and the weight being 



i.e. j forty-eight hours from 

 exudation had occi 



14*64: grammes. Twenty- four hours later, 



beginning of the experiment, a certain amount of further 



rred and the weight was 14*13 grammes, the total loss from the 



beginning 



limitation of exud 



of the experiment having only amounted to 6*8 per cent. 



The 



attending 



exposure to thi 



reagent 



as compare 



vness and 

 with the 



discharg 



taking place under the influence of the oth 



is very 



arkable 



Th 



phe 



must be d 



ther to some alteration produced in the cell- sap, whereby 



stable in place of unstable 



ma 



come to be present in 



or more probably 



to alterations in the protoplasm rendering it 

 circumstances. *" 



less filtrative than it is under ordinary 





Exp 



A leaf of Kalanchoe, weighing 26*87 



grammes 



j 



was set in an 



ammonia chamber. Conspicuous sweating took 



in the lobes nearest to the vessel 



taining the 



monia within the course of five minutes 



assumed an intense deep g 



colour 



and the tissues gradual!} 



green 



The surface of the leaf was moist, but its textur 



On the following day it was of a very deep olive 



not flaccid. Twenty-four 



hours later the colour remained as before, and the weight was 23*42 grammes, equ 



to a total loss of 12*8 per 



Experimen t 



A leaf of Kalanchoe* weigh 



* © 



© 



8*7 grammes, was set 



moist 



ammonia chamber. Within ten minutes it became of a vivid deep green, and had be<nm 

 to sweat conspicuously. On the following day it was dark green and very moist, but still 

 rigid and smelling strongly of ammonia. The ammonia was removed so as to convert the 



chamber into a simple moist one and the leaf replaced in it. The weight of the leaf at 

 this time was 8*52 grammes. It was kept under observation for three more days, during 



which it continued 



g 



off ammonia 



chamber, which was renewed daily. The 1 



enty-four hours were 0*88, 0*29, and 0*11 grammes 



indicated by the smell of the water in the 

 osses in weight for the successive periods of 



> 



g 



total loss for the 



experiment of 1*56 grammes, or 17*9 per cent, of weight 



Experiment XXIII.— A leaf of Kalanchoe, weighing 29*22 grammes, was immersed 

 for forty minutes in a 2*5 per cent, alcoholic solution of corrosive sublimate. When 



removed and gently dried, it weighed 29*3 grammes.. 



It 



chamber, and shortly began to sweat actively. 



was now placed in a moist 



On the following day it was partially flaccid, 



much fluid had been already discharged, and active exudation was still 

 this time it weighed 26*44 grammes.. It continued to 



going on. 



At 



discharge fluid, although kept 



eight days, and at the close of 



continuously in a sealed moist chamber, for the next 



that period it was excessively flaccid, of a pale yellowish olive colour, and only 

 grammes, corresponding to a total loss of 8*19 grammes, or 28*2 per cent. 



weighed 21*03 

 of weight. 



in a 



_ V 



Experiment XXIV. 

 saturated aqueous 



A leaf of Kalanchoe, weisrhin"* 



120 



of corrosive 



grammes, was immersed 



freshly divided after immersion in order to facilitate absorpt 



blimate, the extremity of the petiole being 



Twenty-two hour 



■ 



* 



