IN THE MOTOB ORGANS OF LEAVES. 



29 



exudation than the chloroform, but the action 

 sufficient loss had occurred to induce appreciable 



the chloroform, 

 steadily. On the 

 perfectly flaccid and 

 The loss of 



hen 



dation had 



following 



day tl 



ie 



appeared very soon to be arrested before 

 loss in turgiditv; whilst, in the case of 



nee been established it advanced rapidly and 



f a was of a pale yellowish olive 



psed 



weight which had 



1st b was deep dark 

 rred in a was 7' 7"> 



d 



qu 



grammes, 



dation was still taking place. In the case of b the loss only amounted 



live colour and 

 firm and turgid. 

 27*4 per cent., and 



to 1-C4. 



5*9 per cent., and there was no sign of any continued exudnt 



Subsequently 



b only was kept under ob 



v 



being weighed at intervals and having a fresh petiolar 



face exposed each time that it was returned to the 



mb 



r 



.ty-f 



I) 



bsequently to the previously recorded observation, in place of showing any lo>s of v 



the initiation of the experiment the 



it showed a 



gam 



of 0*42 : and a week aft r 



eniained highly turgid, and the total loss of weight for the 



period only amounted 



to 1*64 grammes 

 following table: 



5*9 per cent. 1 



ghts at different periods ar< shown in tho 



Date. 



t 



February, 13th 



>* 



99 



If 



5> 



5» 



y> 



14th 

 15th 

 10th 

 17th 



18th 

 20th 



• • • 



• t • 



• + • 



• • t 



• • • 



• • • 



• « • 



§1 * 



t • . 



• «• 



• • • 



• • • 



Weight in 

 grammes. 



27-54 

 2;3 90 



26-31 



26-31 

 26-32 



25-90 



Total lose. 



• •• 



1G4 

 1-22 

 123 

 1-23 

 1-22 

 1-64 



Ferottitag" 

 loss. 



• ♦ « 



6-9 

 44 



4-4 

 44 



4-4 

 59 



Up to 



16th the leaf 



r 



ned continuously 



ch 



hut it was 



then removed and kept in a simple moist chamber during tho rest of the experiment 

 When removed it had a very strong ammoniacal odour, and for several days it continued 1 1 



gi\ 



off enough ammonia to endow the water in the 



with a 



S 



ssel of the mois 



ber 



smell of it. Whilst 



this chamber it discharged a considerable amount 



of fluid from the terminal lobe 



happened to be sloped 



abruptly downward 



the 



dation beginnin 



appear 



soon after transfer from the ammonia chambe 



and 



teadily until the end of the experiment, in spite of the u 



The fact that an actual increase in 



eciabl 



loss 



in 



weight" which accompanied it. The fact that an actual increase in weight manifested 

 itself during the course of the second twenty- four hours of tho experiment is remarkable, 

 and is probably to be ascribed to a continued absorption of ammonia and increase in 

 osmotic property of the cell-sap during that period partially making good the initial 



loss of natural osmotic property which had attended the death of the 



The 



fi 



addition of ammonia to the 



sap 



does not suffice to maintain the osmotic 



of the latter at quite the normal level, but it prevents the gr 



property attending the 



of functional activity from produ 



the effects which 



it 



ally would give rise to. The loss in functional osmotic property appears to 



; and hence an 



proceed more rapidly than the acquisition of artificial osmotic property; 



initial fall in turgescence below the level at which the artificial property, when fully 



developed, is able to maintain it. 



Experiment XX.— A leaf of Kahnchoe laciniata, weighing 27*13 grammes, was set in 

 a moist ammonia- chamber with the base of the petiole, which had been subaqueous!) 



