

30 



THE CAUSES OP FLUCTUATIONS IS TUEGESCENCE 



divided, immersed in water 



Twenty-four hours later it weighed 25*94 grammes 



> 



e- 



ponding to a loss of M9<\c. of fluid, or 4*3 per cent, of total weight. The weights 



for 

 having 



follows : 



the first six days of the experiment during which 



kept in the original chamber, 





the petiole freshly subaqueously divided every day after weighment, were as 



Date. 



March, 2nd 



v 



» 



» 



*> 



» 



3rd 



4th 

 5th 

 6th 

 8th 



• • • 



• • • 



# • • 



• »• 



• •• 



"Weight in 

 grammes. 



27-13 

 25-94 

 25-96 

 26-16 

 26-21 



26-21 



Percentage loss in 

 total weight. 



4-3 

 4-3 

 43 

 3-0 

 3-3 

 3-3 





After the first 24 hours, in spite of the fact that it daily discharged a large quantity 



of fluid, an actual increase in place of any further decrease in weight occurred 

 certain point, the tissue at the same time remaining excessively moist and turgid 



up 



to a 



On the 



8th of March it was transferred to a 



loner 



immersed 



pie moist chamber, and the petiole was no 



which the 



ghts were as follows 



It remained in this for the next six days, during 



Date. 



March, 9 th 

 „ 10th 

 „ 11th 

 „ 12th 

 „ 13th 

 „ 14th 



• •• 



• • • 



• • • 



• • • 



• • t 



Weight in Percentage loss in 



• •• 



• • • 



» • • 



• i • 



t * • 



grammes. 



25-87 

 25-67 

 25-49 

 25-26 

 25-09 

 24-90 



total weight. 



4-6 



5-3 



60 



7-2 

 7-5 



82 



It was now once 



with the result that 



gained considerably in weight 



more set with the freshly divided extremity of the petiole in water, 



m to discharge water actively and at the same time 



it again beg 



shown below 



Date. 



March, 15th 



» 



» 



« 



16th 



17th 



18th 



• t 



• •• 



• %• 



• - • 



• »• 



• • • 



• •• 



Weight in 

 grammes. 



25-72 



25-62 

 25-64 

 25-63 



Percentage loss in 

 total weight. 



51 

 5-5 

 5-4 

 5-5 



This experiment is specially instructive on several grounds. It affords a most 

 perfect demonstration of the fact that, even in those cases in which the maintenance 

 of turgidity is normally dependent on continued vitality of the protoplasts of the 



tissues, the rel 



between the two cond 



any 



ructural peculiarities of the 



living 



protopl 



is not a direct one determined by 



but indirect and maintained 



through the intervention of the presence of certain osmotic properti 



in 



tl 



The protopl 



of the tissues of this leaf had 



cell-sap 



tainly been killed on the 2nd of 



March, and yet turgidity persisted in high degree sixteen days later, without there 



bein 



g any obstruction to the free 



passage of fluid 



through the 



tissues, as was clearly 



