321]  STUDIES ON MYXOSPORIDIA— KUDO 83 



entiated into ectoplasm and endoplasm, the whole protoplasm showing 

 granulated reticular structure. Cysts contained numerous fully developed 

 spores and a small number of spores in developmental stages, which sug- 

 gested the fact that two spores rise from each pansporoblast. The parasite 

 is also found in the state of diffuse infiltration in the connective tissue 

 around the cyst. Diameter of cysts 316 to 445/i in sections. Polysporous. 

 Spore: In front view, isosceles triangular form, two sides of which 

 usually convex, with more or less attenuated anterior end (Figs. 87, 90, 

 92); in profile, ellipsoidal (Fig. 88); and oval viewed from the anterior 

 end (Fig. 89). Sutural plane at right angles to the longest diameter (Figs. 

 87 and 89), Shell comparatively thin except at the anterior end and has 

 many fine network-like ridges on the surface. These ridges are hardly 

 observable on fresh spores on account of their fine form and the conspicu- 

 ously large polar capsules lying in the spore. When stained, however, they 

 are not only made distinctly visible, but the prolongation of each ridge 

 from the posterior edge wbiph forms about l/x long fringe-like structure is 

 also more clearly recognized (Figs. 90-95). Two large and spherical polar 

 capsules located in the central portion of the spore. Coiled (5 to 6 times) 

 polar filament extremely distinct. The openings of polar capsules at the 

 anterior end. Sporoplasm comparatively small, finely granular, without 

 any vacuole, contains two small nuclei, when stained. Dimensions in vivo: 

 sutural diameter 9 to lO/x, breadth 10 to 12/i, thickness 6ju, diameter of the 

 polar capsule 4/i, length of polar filament 35 to 45/x. 



WARDIA OHLMACHERI (Gurley) Kudo 



[Figs. 96 and 97] 



1893 Myxosporidian Ohlmacher 1893 : 561-567 



1893 Chloromyxum ohlmacheri Whinery 1893 : 660-662 



1894 Chloromyxum {Spkaerospora) 



ohlmacheri Gurley 1894 : 267-272 



1895 ? Leptotheca ranae Th61ohan 1895 : 383 

 1899 Leptotheca ohlmacheri Labh€ 1899 : 87 



Habitat: Urinary tubules of kidney of Bufo lentiginosus Shaw and 

 kidney of Rana esculenta and R. temporaria {R. fusca) ; Sycamore, De Kalb 

 county. 111. 



Vegetative form: Not found. 



Spore: Transversely elliptic. Sutural plane perpendicular to the longer 

 axis of the spore. A well defined undulate-parallel longitudinal striation 

 on the shell. Sutural ridge comparatively well marked. Two polar capsules 

 lying side by side, occasionally only one. Dimensions: sutural diameter 

 6ju, breadth 8/i, diameter of polar capsule 3 to 3.5/:, length of polar filament 

 6 to 8 times the breadth of spore (48 to 64/i). 



Remarks: This form is apparently very much different from any 

 species of genus Leptotheca, in the general form, form of polar capsules, 



