84 ILLINOIS BIOLOGICAL MONOGRAPHS [322 



striations on the shell and the habitat. Tho the form of the spore is differ- 

 ent from the type species of the genus Wardia and nothing is known about 

 the vegetative form, the presence of large spherical polar capsules in the 

 central portion of the spore, the striations on the shell and the occurrence 

 of the same nature, i.e., from fresh waters in the close-by localities, show 

 its nearer relationship to the genus Wardia than to the genus Leptotheca. 

 Hence it is placed here provisionally. 



Genus MITRASPORA Fujita emend. Kudo 

 1912 Mitraspora Fujita 1912 : 259-260 



The characters of the genus are described on page 56. 

 Type species: Mitraspora cyprini Fujita. 



MITRASPORA CYPRINI Fujita 



[Figs. 98 to 104] 



1912 Mitraspora cyprini Fujita 1912 : 259-260 



Habitat: Renal tubules of kidney and ureters of Cyprinus carpio L. 

 and Carassius auratus L. ; Sapporo (winter), Tokio (March). 



Vegetative form: Fujita's only description is as follows: "The sporo- 

 blast contains generally three or four spores." The present writer observed 

 a similar form in the ureter and the renal tubule of the kidney of Cyprinus 

 carpio L., in Tokio, The observations are as follows: Trophozoites small 

 ameboid (Fig. 98). Body colorless. Movements tardy. Differentiation 

 of protoplasm imperfect. The hyaline ectoplasm recognizable at one side 

 of the body, where lobose pseudopodia are formed (Figs. 98-99). Endo- 

 plasm granular with vacuoles and brownish granules, which become 

 larger as the body grows. Size 10 to 40/i. Disporous (Kudo) and poly- 

 sporous (?, Fujita). 



Spore: Fujita's descriptions are as follows: Form resembles monk's 

 hood, slightly attenuated at its anterior end. Shell uniformly thin, except 

 at two points of the truncated posterior end. Each shell valve has eight 

 distinct striations which run longitudinally and turn into long cilia up to 

 5.8jLi long, planted in a single row at the posterior end of the spore. Two 

 polar capsules at the anterior end. The nucleus is obscure and no vacuole 

 is present. Dimensions: length 10 to 13/x, breadth Sn, polar capsules 

 3.8/x by 2n, length of polar filament 15/i (weak glycerine). The writer 

 observed the following facts: More rounded with rounded anterior end in 

 front and side views. Shell more or less thick along the entire posterior 

 margin. Striations on shell, variable in number. Sporoplasm granular, 

 without any vacuole, exhibits two nuclei when stained. Posterior filaments 

 5 to 6 in number and 5 to 6/i long, being absent in some spores. Dimen- 

 sions in vivo: length lO/x, breadth 8 to 9/i, thickness 6 to S/x, polar capsule 

 4n by 1.5 to 2/i, length of polar filament 35 to 40/i. 



