96 ILUNOIS BIOLOGICAL MONOGRAPHS [334 



CHLOROMYXUM GRANULOSUM Davis 



[Figs. 157 and 158] 

 1917 CUoromyxum granulosum Davis 1917 : 237 



Habitat: Urinary bladder of Tylosurus marianus; Beaufort (July, 

 August). 



Vegetative form: Elongated when first placed on the slide, but soon 

 becomes contracted and motionless; progressing by very slow ameboid 

 movements. Ectoplasm usually undistinguishable, being noticed only in 

 a few individuals which had formed one or two short, lobose pseudopodia 

 of hyaline ectoplasm. Body colorless to light yellow. After being on the 

 slide for some time rounded trophozoites often became surrounded by a 

 distinct ectoplasmic layer. Entire body usually coarsely granular, the 

 granules var\dng greatly in size and shape; sometimes indistinctly vac- 

 uolated. Fat globules also present. Size of rounded trophozoites about 

 30/i. Disporous and polysporous. 



Spore: Spherical, with four distinct ridges on the posterior half of 

 each valve converging toward the anterior end. Sutural ridge distinct. 

 Polar capsules pyriform and convergent. Dimensions: diameter 7/i, 

 polar capsules 2n. 



Remarks: Trophozoites from some fish were" all colorless, while the 

 larger trophozoites from others were distinctly yellow. 



CHLOROMYXUM TRIJUGUM nov. spec. 

 [Figs. 159 to 182] 



Habitat: Gall-bladder of Lepomis megalotis Raf.; Stony Creek, and 

 Homer Park, HI. (November). The parasite was only found in this species, 

 Lepomis humilis and L. cyaneUus seined at the same time being free from 

 the infection. Six specimens, three from each of the above mentioned 

 locaUties, harboured abundantly both free spores and trophozoites of 

 various stages of development in the bile. The fish, from 6.5 to 10.5cm 

 long, were normal in external appearance and the bladders did not show any 

 particular abnormality, compared with those of other fish, as is usually 

 the case. 



Vegetative form: Trophozoites float usually free in the bile, younger 

 forms are most frequently attached to the epithelium of the bladder. Form 

 extremely polymorphous, manifesting various shapes such as, almost circu- 

 lar, rounded, oval, elongated or irregular, which is chiefly due to the active 

 extrusion and retraction of the pseudopodia from the body surface. Body 

 is highly transparent and colorless in both the young and the adult. The 

 differentiation of protoplasm into ectoplasm and endoplasm, is distinctly 

 visible in vivo as well as in stained preparations, especially in larger forms 

 (Figs. 159 to 165). The endoplasm presents an alveolar structure without 



