3551 STUDIES ON MYXOSPORIDIA—KUDO 117 



MYXIDIUM KAGAYAMAI nov. spec. 

 [Figs. 294 and 295] 

 1916 Myxidium sp. Kudo 1916 : 6 



Habitat: Gall-bladder of Misgurnus anguillicaudatus Cant.; Tokio 

 (September), 2% of the fish examined infected. 



Vegetative form: Not observed. 



Spore: Fusiform; one valve being more convex than the other. Suture 

 line straight. Shell with fine longitudinal striations. Dimensions in fixed 

 preparations: length 15 to 18m, breadth 6 to Ijx, length of polar capsules 

 7 to 8m, length of polar filament 60 to 70^. 



Remarks: Tho the vegetative form is still unobserved, the author is 

 compelled to consider the present form as a new species by careful 

 reexamination of the material and proposes the name in honor of Dr. 

 T. Kagayama, Tokio, Nippon. 



MYXIDIUM AMERICANUM nov. spec. 

 [Figs. 622 to 627] 



Habitat: In the lumen of urinary tubules of the kidney of Trionyx 

 spinifera; Crystal Lake, Urbana, 111. (July). A single host specimen 

 showed a light infection in the above mentioned organ. No intracellular 

 stage was detected. 



Vegetative form: The young trophozoite in the lumen of the tubule 

 of the kidney is multinucleate, and more or less irregular in shape which 

 suggests the ameboid movements of the animal (Figs. 622, 623). The 

 older form with mature spores is rather spherical in form with a distinct 

 outline. The protoplasm is fairly well differentiated into ectoplasm and 

 endoplasm (Fig. 624). The size of the trophozoites varies from 12 to 25/1 

 in diameter. A pansporoblast produces two spores. Polysporous. 



Spore: Spindle-form; with the two pointed extremities stretched in 

 opposite directions. Circular in cross-section. The shell is rather thin; 

 sutural line is straight. Fine longitudinal striations on the shell, eight 

 to ten in number on each valve. The polar capsules are nearly spherical, 

 coiled polar filament being visible in fresh material (three turns). The 

 polar filament is easily extruded from the fresh spores under the influence 

 of potassium hydrate solution. The direction of the extruded polar fila- 

 ment forms an angle of about 45° with the main axis of the spore and the 

 two filaments are parallel to each other. Preserved spores do not show 

 any filament extrusion under the influence of the said chemical. The 

 sporoplasm is finely granular, and shows, upon staining, two small nuclei 

 of ring-shape, as their peripheral layer takes stain more deeply than the 

 central portion. Average dimensions of fresh spores: length 15 to 16m, 

 breadth 5.5 to 6m, polar capsule 4m by 3.5m, length of polar filament 

 25 to 32m. 



