393] STUDIES ON MYXOSPORIDIA—KUDO ISS 



or more in water, the polar filaments are extruded. The binucleated 

 finely granular sporoplasm shows an iodinophilous vacuole. Dimensions: 

 length 12.4 to 13.5/i, breadth 6.5 to 7.5/*, thickness 5/i, length of polar 

 capsule 6 to 7/i (rarely 7.5/x), length of polar filament about 20 to 26/i, 

 diameter of iodinophilous vacuole about 2n. 



MYXOBOLUS MIYAIRII nov. spec. 

 [Fig. 481] 

 1909 Myxoholus sp. Miyairi 1909 : 130, 131-132 



Habitat: Intestinal wall of Parasilurus asotus L.; Fukuoka ? (Nippon) 



Vegetative form: Cysts. Size rather small up to 0.5mm. Full-grown 

 spores as well as those in developmental stages fill the central portion of 

 cysts, while numerous nuclei are chiefly found along the periphery of end- 

 plasm. 



Spore: Elongated elliptic. Two polar capsules of nearly same size. 

 Sporoplasm with a comparatively large iodinophilous vacuole. Dimen- 

 sions: length 13 to 14.5)u, breadth 6 to 7/x, length of polar capsules 4.5iLt, 

 length of polar filament 30 to 35/i. 



Remarks. As the descriptions show the form and structure are dis- 

 tinguishable from other species, the writer establishes the present species. 



MYXOBOLUS KOI nov. spec. 

 [Figs. 482 to 485] 



Habitat: In the connective tissue of the gill filament of CypHnus carpio 

 L.; Tokio (April). One fish was found infected in a slight degree. 



Vegetative form: Cysts small and spherical; white in color. Size up to 

 230m in largest diameter. The seat similar to Myxoholus toyamai. The 

 structure of the cysts, observed in section preparations, is also similar to 

 the above mentioned unicapsular Myxoholus. 



Spore: Oval with attenuated anterior and rounded posterior ends in 

 front view; elongated pyriform in side view. Shell comparatively thin. No 

 marking on shell. Sutural ridge fairly well marked. No intercapsular 

 appendix. Two polar capsules are pyriform, large, and of usually equal 

 form and size. Coiled polar filament distinct in vivo. Sporoplasm rather 

 small, finely granular, shows two nuclei in almost all spores. An iodino- 

 philous vacuole is deeply stained by Lugol's solution. Dimensions: 14 to 

 16m, breadth 8 to 9fx, thickness 5 to 6m, polar capsule 8 to 9m by 2.5 to 3m, 

 length of polar filament 72m in average (KOH). 



MYXOBOLUS ORBICULATUS nov. spec. 

 [Figs. 566 to 576] 



Habitat: Muscle of myotomes of Notropis gilherti J. et M.; Stony Creek, 

 111. (November). The fish was kept alive in an aquarium from November 



