172 ILUNOIS BIOLOGICAL MONOGRAPHS [410 



Vegetative form: Ward describes as follows: The whitish opaque cysts 

 are pjniform, and fairly uniform in size (3 to 6mm. in diameter). The 

 cyst is covered by numerous layers of connective tissue which form a 

 tough membrane around the parasite. The cyst contains young spores 

 in various stages of development, which showed that two spores are formed 

 in one pansporoblast, and mature spores thickly massed together in the 

 central area. Polysporous. 



Spore: Oval with rounded anterior and more or less attenuated poster- 

 ior ends; elliptical in profile with attenuated anterior end. Shell smooth. 

 Sutural edge exhibits folds variable in number (usually 6 to 7). Tail 

 double, composed of two fine and equal halves which are the prolongation 

 of the shell valves. The processes usually run roughly parallel to each 

 other. Two pyriform polar capsules are of slightly difiFerent dimensions. 

 Coiled polar filament is indistinct in preserved unstained specimens. 

 Sporoplasm finely granular, shows a large iodinophilous vacuole. Dimen- 

 sions of stained and mounted spores: total length 47m (42.75 to 52.44/i), 

 length of the main part Un (11.97 to 14.25>x), breadth 8^ (7.12 to 8.43m), 

 thickness 4.78At, length of tail 35/* (30.78 to 38. 19m), polar capsule 3.70 to 

 4.55m by 1.59 to 2.85m. 



Remarks: Zschokke and Heitz (1914) observed a species from Kam- 

 tschatka, which they thought to be identical with Henneguya zschokkei 

 (page 165). The writer is inclined to think that the species is identical 

 with the species just described from Alaska. 



HENNEGUYA MIYAIRII nov. spec. 

 [Fig. 524] 

 1909 Henneguya sp. * Miyairi 1909 : 127-129 < 



Habitat: Subcutaneous tissue of head of Carassius auratus L.; Fukuoka 

 (Nippon). 



Vegetative form: Trophozoites form cysts and are also found in the 

 condition of diffuse infiltration around the cysts. Cyst-membrane fibrous 

 and thin. Ectoplasm and endoplasm fairly well differentiated, though 

 the border line is not sharply marked. At the periphery of endoplasm, 

 pansporoblasts with 7 to 12 nuclei are present (Two spores are formed in 

 each pansporoblast?). Polysporous. 



Spore: Oval, with broadly rounded anterior and slightly elongated 

 posterior ends, the latter ending in long and fine tails. Two polar capsules 

 at the anterior portion, are pyriform, small and convergent. Sporoplasm 

 with an iodinophilous vacuole. Dimensions: length 12m, breadth 8m, 

 length of the tails 10 to 30m, length of polar filaments 23 to 40m. 



Remarks: As the description gives the details by which the species 

 can be distinguished from other species, the writer estabUshes it on an 

 independent basis. 



