370 



Junes B. Swtuger, Ph.D. 

 March 22, 1994 

 Fhge2 



pcstoaaei oa the taoiple ejtnets just prior to asalysu. fiuicalty. the cooDplete sample was 

 bosoosemztd and a 250 mL glass beaker was filled torn this hoiQo;enin»d sample and diied 

 2t ~96*C for 2 hours. After dzyin^. samples weane itoted ia screw cap plastic bottles and 

 sealed witii Toflon tape until extnction. To extract, duplicate 1 gram saa^tles wete weighed 

 out imo aepn«te 60 mL oectrifuge tubes, \vet with 10 mL of water aloog with 10 mL of a 

 10% todioxn liydioxide solution and extracted with 20 mL of chloroform costalaiag 0.4 ppm 

 aaetbole, the faKcxoal standard. This was accoii]3>Uabed by ^haiHng oa a wrlit actioD shaker 

 tot 20 mloutes. The extract hytat -wcLt then sepanted by cestdAigalion. A portion of the 

 chlcrofbxia Iiyer was removed (~S mL) and a 1:100 dilutioQ In strtigbt chloroform was dooe. 

 The samples were tbeo aa&lyzed along widi calfbratioQ itaodards by OC/MSD tuiog single 

 ioa monitoring for the preaence of nicotine. 



A oonTaiatlve analysis between the two atartiog materials (stem and (cap) to the fioisbed 

 sheet (fical} appears to indicate that over thft time pedod wt sampled the level of olcotlnr in 

 the floa] product we analyzed never exce e ded the sum of the oicotme levels fousd in the two 

 staitfaig maserials. The data reported to date iodjratfd fbat the nicotioe level fax the finisbed 

 sheet it less thaa 50% of the sum of the oicotinc levels found in the two etarthig materials. 



Daring tbe process overview givea by R. J. Reynolds, it was stated that the target 

 percentages of stem and soap oxd to mate tiie finished sheet was 6S% stem and 325( scr^. 

 If that perocatage was met for this final sbutt, then you would expect an average contribution 

 of approximately 1730 ppm aicotiDe from the stem and an average contribution of 2774 ppm 

 finxn die scrap for a total average of 4504 ppm. 



These nambera were estimated using the preliminary average values for stem and scnip found 

 in the spreadsheet atkl multiptyiog by 0.68 and 0.32 regpectively. When you compare these 

 values to the prelimisary average tor the fical sheet of ^^proximately ^963 ppm. these data 

 are In good agreement wbh ei:q>ectEd values. In tttn the preliminary Average for tbe finished 

 sheet is tlighdy Ins than the estimated value based on Ibe stem and scrap averages. 



As wt d iscussed in detail before, these data sull need to go through a complete GLF review. 

 The porpose ot this review is to catch any action items that would cause these numbers to 

 change or cause additioiud work to be done, u well as to enntre that you receive a quality 

 product under die GUP guideliaos that w« followed throo^ioat this project. Until the full 

 OLP review i« oomplete, these data can only be considered prelimisary. Any otber use of 

 these dati at this time is considcied tbe responsibility of ttie R. J. Reynolds Tobacco 

 Company. 



iy fbcos now win be on preparing tbe final npocL If yoa have any questions about these 

 dila, please don't hesitate to can me at (61^ 424-4190 or ftjt me at ((14) 424-3204. 



Sincerely yoots, 



Stqibca J. Summer 

 MMter Research Teefanieian 

 ScddDA and Praduet Cbemiscty 



