508 



aoekltis will b« oendueied later and citeapts u0« to 0«llna«te alftiltritles 

 •Ad 4irf«r«no«a la nleotln* pharaaceld.Beties tt\*r aoutc and chronic ust 

 of aaokad tuad aaokel«*a tdbaeeo produota. 

 ttATERIiLS AMP HTTHODS 

 lleaiwnta 



(•-}>KlaetiM, purohascd froa CaatMn Kodak Co., vts purlflod by neuia 

 tflatinatlee (123-125^) *nd atorad uodcr nltrcBan. {.}-eetliila« waa a gut 

 rro« B. MtfaoBla, Madleal Cellace ef TlrflaU and (-}-SleotlM-V-««<aclda 

 vaj ajrDthetlaed aeeerdlng to PhllUpaen & Randa (1975). Kodsllna sulTate 

 (2-«etl))rl-3-plpcridln»-p7raalDe] waa ebtaloed Troa Vara«iM.«Bb«rt Zastltute, 

 H.J., while prllecalne hydreehlerlda, froa which the free base was (aaerated', 

 was supplied by Utrm Pbaimecutical Prods., Kass. Titataua trleblorida 

 (t«ehatoal grade) aolutieo, 20i was purchased from Baker Chealoal Co. and 

 diluted to e.2K by eeablnlng 1.5 parts of It with 1 part ceBceatratad BCl 

 end 7.S parts dlatllled water. Other reas«nts and >el vents used and their 

 seurees are Hated below: 



Ether, abaolute reagent - Hatbesea, Colenan ( Bell, Norwood, Ohio 



Methylene chloride, Spectranalyxed - Timber Seientirie Co., Pair Lawn, N.J. 



Butyl aeetatc, Photrez reagent - Baker Chee. Co., Philllpsburs, N.J. 



Acetone, glass distilled .- Burdlek A Jackson Lab Zae., Muskcsen, W 



Sodlva hydroxide, lOM - Flaher Sdeatiric Co., Pair Lawn, H.J. 



SediiB ectablaulflte - Flaher Soieatirie Co.. Fair Lawn. H.J. 



Hydrochloric aeld, cooeentrated (IZ.ZN)^- Baker Chealoal Co.. PMllip»burg, N. 



Subjectj and Saaplcs 



Six naive and sl> habituated ciale tobacco snurr takers took part In 

 the study. The habituated subjects eonsiBcd tobacco snufr en a regular basis, 



"CONFIDENTIAL" 



"This dotumeni is svMect lo « protec- 

 tii't either tnd ntllW il ftoi its 

 CMteots nuf be shoani. discusied or 

 diKlosed eicept ia tccsrdanct villi udi 



