798 



Kleotine. 



Vaighed sets of 10 cigarettes (as received) were treated bj 

 the A.O.A.C. insthod for nicotine in tobacco and tobacco products 

 (Methods of Analysis, 6.108). Results were corrected for the moisture 

 content and for the paper weight, and reported on the dry basis. 



Smoking Teats . 



Sets of 10 conditioned cigarettes were saoked in the automatic 

 apparatus previously developed for reproducible sacking conditions, 

 each cigarette requiring about 7 to 9 ainutes to be snoked to a 23 nn. 

 butt. The aspirated smoke was drawn through a series of four separatory 

 funnels each containing 12.5 oc. of chlorofora and 12.5 ec. of i% sul- 

 furlo acid, at half idjnute intervals, each "draw" being about 2 seconds 

 duration. After the 10 cigarettes had been smoked successively, the 

 chlorofom extract, including washings, was evaporated and dried at 

 100* for 1-hour periods until the loss was less than .005 gran. The 

 separated acid, aqueous solutions, and washings were used for nicotine 

 determinations by ths A.O.A.C. asthod. 





