828 



QuuconoNioea or NicoiiNe and its metasolites 



195 



3 = y>*rironfZOlfmt. C - Codnnc N c ^«em* 



F\(i. 2. Diurtbuiion o/mnj'jr winary nlaxine metafxHiCrt In It smokers. (Each group vfthiix Hsrs rzprnemi data from oat jmckfr.) 



Ra 3. Avtrate JiMntuliun vfmnery rjootine me(abolUafor 1 1 jmuktrs. JIIC. traKS-i'-hvdmiiaxMnc COT. coaidnc NIC nloMlne: UNO, 

 nianins^-aidii; CNO, Mlnint-H-axUa: MIC, ikmtikyleMimlHt. '-G' Indicatn iht gluc%maidi etu/igalc <^lfiai metabolite. 



TABLE 2 

 TiAai emourU efnicalme excrtud In a 14 -hr urine sainfU by oncken 

 at lilcstint end ilt ma/ar matahclhtt 



Ssioker 



Tcnl 



FTC 



Njuxiu 



VkU 



Tyjsl 

 Nioociae 





' Tai*l FTC MxnsAc yiddcd fr^tn fS\ cisaiTsici ooiaumad duiia; U*c 

 2<-h» palod tatal oo Uic yield putjlimea la r«f. 13. 



*$u(n of *B anotry mojibollus detetminal ncrrraliwd (o nicotine 

 cauivftleaU &i dc«3Tbcd in the Ualjriatt tu^d .Utithodt. 



'Rittio of nigQtioc »quiv&i«at« A&orvtcd (out) tc r7C ai«o<>ne yield 



(in). 



co^jugatfs. The avenge nlica tKtween 6«: and coQju£Med 

 tllutltsadi of nicotine, colinlne, tod (rani>3'-hydroxyo3tl(iiat tr. 

 our study were 4,0, 0.?, atKl 4.0, rnpcoivdy, wberMS Cutvtli 

 fcoortcd ratios of 1 .0. OjS. awl 13, respectively. Thus, we round 

 cnucb Im6 of the ooqjueated Iotbu of tlicae (iu~t mctabolita 

 Uiait CuroU reponed. In tarns of total usctLois of frte «sd 

 cotijue:ited fonos of these mtjcr thn* in«taboih«s in cmaken, 

 wc find 2.6, 6.7, lai. 1 l.d mg pv TAAtt uilnc umplc for oloodne, 

 cotiailK. aixl /r^i/tf-3'.hydroz]^:otiiiiBe, ze^eaivAy, eomiured 

 with 2.1, 4.9, «nd 13.0 in the Cu.-ttiII study. The diircrtaocj in 

 gjumronids piopanioa bctvvca: our resulb xa& CurviU'i may 

 reflect individual diCfef co eet ia nieotina mstabolicm Of m(y (m 



due 10 difii^noa m ttic aaatytlcal iccimlques. Our meOiod uses 

 a iS^dttCuromdas: treatment cmiUr to CurvaD'n. bat tlie inalyiii 

 for nioouoe, (joosioe, aad /ran<-}'4tydcoxyootiniiic ii diflixeaL 

 Oar method require] oo C7.li%:tion or doivatixation, u was lued 

 by Cunill for the detenninttion of the Ene sjtalytM. In foci, 

 tiumao tninc i> icjcvtcd dinztly on tlie LC fSUOwinj uiln(lm> 

 tiooL This mininirzzs the poteatiai for bu of munwl durieg 

 caicple prepantioa end reduces the potential for ihcmid dcoom- 

 puidtioo. 



Our eflbrts to detect glucumnids canJMfA'JX dic^Sly utiog 

 thcnnoapray LC/MS in the man nn^a that would be expected 

 were uas'jcocaftjl. We were able to dctaci three mode! alucuro- 

 nide cCQJugntes' ;?-a*phth-/lg](unjpooi<to, C-bydro^j^quinolioc 



