430 



Wtkohi 0t d.—B*perimenlal CcrcinoiM wiU CifnO* Ttr 



uma c*rciao|eo, » preant la to b a cc o, bat Ncnt 

 ftadicJ by Dtff tad co-workeri (I) baJed ob tb« 



•jwoie coabrot oi vanouj t/pei of EoropeAn 

 tobacco Undi to pUc« lea eapbMif oo t^ laor> 

 ftok element Heat, cigvettc paper. fUvoni|. tad 

 vetting AfCbU havt baea Nggcftcd w etwlofie 

 fuciort b tbc prodtMtioa of cai»oer. bat it «»Q«t b« 

 soled tbat clinical evjde&ce bJu alao poiatcd to 

 cigar-naokiog, pipe-iniokiof, aad toba«o-ebrw- 

 h^ a« potabk (actors ia tbe production of eaacer 

 of tb« re«pintoi7 aad aOmentaxy tract 



Tlie actual caKioogeme agtat or acentj 'a to- 

 bacco KQain to be idenliiled. Studiej combiaiDC 

 cbemJcaJ aad biologic effort* leading to l^oir >d«o- 

 tification are n/geDtl/ needed SbouJd one be able 

 to identify definite carriaogeoj aad lueceed o> re< 

 moTug t}>etn, or at kaft m reducing tbei/ quaAtity 

 in tobacco, proper preventive toetbodj would be at 

 baod. Such JtudJej may furtber our ocderttaoding 

 of buinaa and animal cardnogeneaii and inay lead 

 to tbe developnyeot of practical preventive mcaa- 

 ures agaiait caooer. 



SUIQLUIY AND CONCLUSIONS 

 I. A cigarette tar coodesaat* wai oblained vitb 

 a nsoViDg maciuM whicb timulaled bumaa raok- 

 iag babiti. Tbe rerilUng tar waa dlnohed ia aoe- 

 tooe and applied to tbe backt of CAPi mice is a 

 doaagQ of 40 mg. of tar/acetoD« tolotioQ i timet a 

 w«ek. Coatral mio* were paiated vitk acetoaa. 



t. Of 81 tarrtd mice, 0t per cent drvek>pcd 

 papiDonua. Tbe firvt lesion wai noted ra tbe SSd 

 week, and tbe mean time of appearance vu M 

 weeks. 



$. Of 81 tarred mice. M ^t cent developed kia- 

 toIoficaOy proved earcinomaa. Tbe firtt careiaotaa 

 waa obaerved ia tba 41d vedc. aad tba avengt 

 tkoe of appearaaoe w«« 71 veeka. Of 91 mica aSve 

 at If DQontba, Si per cent developed eancce. Sevea- 



S-ot* weeki oookitutei approfimatHj oae-balf of 

 • Ufa ipaa of CAPi miea. Tbii oorreipaadt 

 rou^ilj witb tbe fact alreadj aotad tbat ia tba 

 btunan about 90^45 year* of aaokiaf. «r approi- 

 tBately ooe-batf tbe fife ipaa. ara laqaliad far tW 

 pro«)Qctioo of broachkfenic eaidaoaa. 



4. One earemetna va* traarplaatad for 4 feBcr* 

 atk>oa aad aaotber oae is currcBtly growiag ia tkt 

 IStJi generatioe. 



g. Control mice painted witb acetooc akme 

 abowed no ilda ksioas. At tbe end of tO moatbi of 

 painting, &9 per cent were still living, compared to 

 08 per ci-nt ia tbe group paiated with tobacco 

 tart. 



6. Tbe group of mice painted with crotoa oil ia 

 addition to tbe tar, starting ia tbe 7tk mootb, 

 cannot be properly evaluated because of a greater 



Doaber of deatba oecwnag dnriag tba Utb aad 

 14th montbi, ahboQgk witbja tbe period of obser- 

 vftiioa no icceleration of cancer fonnatioa was 

 noted. 



7. Tbe group of mice started witb aeetoM and 

 receiving erotoe oil begiaaiag ia the Ttk aoatb 

 sboved rou^eaing and tbickeoiag of tbe cp^ 

 dermis, but ito ttunor focmatioa was noted. 



1 AA CA?i Bijoe painted witb OJ pe cent 

 •olutioa of metbrlcbolsatbrcoe hi •oetooa drvvt- 

 oped cancer whbb 4) mootbs 1^ Sr^ papiBerna 

 appeared during tbe 8tb week, vitb aver&gt ap> 

 peaxaoce during tbe 7tb leek. Tbe flnt carei- 

 ootna WW obaerred during tbe Ittb week, witb a 

 mean time of appearazMe of 19 week*. 



9. Tbe resuHi obtained with CA?i mice estab- 

 lisb condeujed cigarette tar at a carcioogq for 

 iriouse cpid«rmi*. l^kcae ttudlM provide a tool to 

 detennioe and isolate tbe possible cardoogenie 

 ageijt(i) within tobacco tar. kX. prejent It is not 

 known wKicb fractioo or fractions in. tobacco tar* 

 are carcinogenic Combined chemical and biologic 

 studies are now rn progress to search for aicb 

 agents. Such itudiea, in view of tbe coroQary eSoi- 

 cal data relating nrtoking to various types of caa* 

 cer, appear urgent. Tbey may resuH not only la 

 furthering our knowledge of carcinogeaesis, bat ia 

 promoting Ksae practical aspects of eaacer pta- 

 vcntioB. 



AOCNOWLSlXiMXNTS 



TW ttUtfi TiA U oryrw Ottk gnUiAt U Caka 1. 

 Cook. Va/? C«j Cnv, %ai C^iKnia Ciaa) o< Wukjagtoa 

 L'aj'inHy Sdioai <A MadkiM, 0«p«rtiMat it hgptji it- 

 Lmia Me., ht tWir t<rknir«1 uoAtaaa 



S£FER£NCBS 

 1. BaswAt, L tU VkUoIb if Cinhnyirfr. A 



Stvdy «f tW S«nJScaaec tl C»«ueiBegab A^tiaa mi 



lUUtad nfoawmt. Caaevr B««MK^ l:l0r-U IML 

 t Boooi. I . v4 Vtotaa, %. N. TtUcea 1W: Aa li»ii»> 



«wta] Lif««>c>UM «f lu ll h |w l C ud mo p tk LdSm^ 



Km. I. Cmcw, 14:1*1«-«1, ISM. 

 1 BaB«o«.LDwiagaietuSMUa|CsaHUagCacw? 



C40«««i*'* BakK ^l-H IMI. 

 4. iaosea. A. TUamJeW ia a ^ nj a i uai t W li n i i Aw 



mm m M> ■!«■* «4 He»i^wwb im ■ilfiM 



C»»Aw<h» WtaeM Aick ftf^ik. hmt. a. FV^. 



a C«xnau.;.A.TWStwUeftikaMtCMMfa«silMw. 



nai Covbvtioa ffncis^ aa<l of TbbMM Smk* f^m 



Mk«, viU S^eaal lalweBe* to labdMB* W Tmmn «f 



tW Luas- Bm. i. Ecpar. PaU, lt:l4«^Ml ll*a 

 a . Caitiaamic .VgMts fi a i at la tba AtMaipkM 



aa4 Inddoca J trmuj Laa< Tweua la Mka. AU., 



«):lia-9«,llM. 

 T. Coom, K, A: Lajo, t. W. U.; Samaaa B.: tad Boar, 



B. L. TK« R«la at TokaMa SMkiB« ia thi fnimtim W 



Caaetf. ;. Hrr. 0:IH KM. IMt, 

 a D&rv, M. B., bou, S.. aad KcnrAW«r, B. L Caseir »t 



tAa Lwi ia B<WlJeo to T«U(a>. BA. J. Caaew, 1: 1-M. 



11*1. 



