369 



->- 



rtltMt of ttercd hooMM*. Ia4««4, Tlrtu«llr *^^ ^^ iJ> 'i^ •tltci$ of 

 alcoClfH MA bt uUlMt«l7 r«l«C«d to the aeabrAo* tetlrt properties of 



'"^fe. 



Chit coopo 



MetuMll;'; j|«rln< aoj icodr of th« bloloflcal tffoet of olcotlat U 

 It of p« r«iio«M^ la|»orC«oc* to «ccur«tcl]r attest the dote of alcotlec 

 absorbed, lo pre^Uce this objective h«t beea difficult to tchleve. 

 PrlMrlly because of i,£t aeabrase active properties, oicotlne It r%y\i\f 

 lott froa the blood to otliikj; tlasuet. Average pUsu alcocine levcU In - 

 swokers rarely Increase above-3J-50 m/al Irrespective of nicocloe ;l«ld 



-•f.^ of the cigarette or the frequency of tooklu^. To gala an Insight lato 



'■^^ ">). 



the netabolltn and hence the aetaboli^'^f fectt of nicotine in vivo ic 



It r<0^ssar]r to aonltor sequentially the 61>^d Icvcla of alcocine prior 



(o, durfftft^and after exposure. Thus plasma iM^lt of alcotloe aetakeUtes, 



Che ^t« oKli^earance of nicoelnc and aetabollt^^ tSt^lae aad 



hence tM t»te "^^learanee should be Matured. Addf^nalw, where the 



causal relattootht^ bftveen nicotine and Individual blochenlca^, 



physiological or ptychot^ical retpontet ar* to b« Invettigated, aeonte 



Infontatloa regarding iUcotuy dote It cttentlal. ^ 



If thete alas and obj«ctlvy> are to b« achieved, particularly asiag 

 experimental anlmalt, it It SAcessa^to develop methods for taalyst^ 

 numerous taall volume biological •aaplerfor nicotine aod its metabolites. 



Naturally the use of small voluae aaapZjM aecessttttes the use a( 



highly sensitive, reproducible and. If pottible^auantltatlve methods. 



Mo assays currently avallsble vere considered tultaMe to aeet these C 



c 

 criteria. This report describes the developaeot of a aQlro-aethod for ^ 



measuring nicotine and cotlnioc levels. ^Q o 



