370 



•^ 



/r 



MCWOt^ AHP HATtHIMJ 



NlcHlnt w«t lufr^i*^ ^T BDH with • •p«eifiH purity of 98X. T^« 

 purity oP8Bk^a«ttrl«l vat ••••y«d by the •p«etrephoto*«trlc attb*4 of 



Wllllt* (43 a(4 t* 4«««rlb«4 In Appendix B. Coclolo« wa« obtained fro* 



C^ ■ 

 Battel!* (FiSMfurt). Although no tpeclflc purity check wa« made, the 



^^ 

 Material chroaatojfr«ph«d aa a alntlc coaponcnt. *,4-blpyrldyl (99T pure), 



~>> 

 aodlua hydroxide (AAXaad aodlvn oxalate (AX) were all obtained tbrou|h 



BDH. "'*;.. 



V 

 n-butyl acetate (A*), ctiU>j-ofor» (AX) and propan-2-ol (I?A; Ai) were 



^'■. obtained froa BDII and redistilled before ose. 



Ucon HB 2000 and Chroaosorb wcrCaupplled by Chroaatography 

 ■ X V> 



Supjirllct Ltd. A, 



Rn J} concentrate was aupplted by Cheat£4l Cooecatrate Ltd. 



''^ Surety wai tupplled by Merck and pre pa red'^^tff^ ua( ky the pcoccdura 



- ^ o^ ^O^ X 



deacrl^4l() Ap^li B. 2) 



All gaata for ifiC «n«l;rsl* were tupplled by Air Products. 



Ca* Chroaatonr'apKlc Aftj^xtla 



A rackard *26 Mrtci g^ chroaatojrtph tqulpped with a tpeclflc 



nltrogen-photphorut detaetor (Hfo) was used for til nicotine and cottnln* 



•nalyaca. (^ 



The following colt«a and OC condltSoM were used for all routlae 



analyses: i> 



-^* 

 Coluan ; Class; 2a x 1.6 an l.d. coatalolng >J Ucon HB 20C0 plus 6Z 



/.- 



KOH on Chroaosorb AW, 80-100 aesh. The Ucon coluan yat prepared asd 

 conditioned according to the aethod described In Appendix A. \ 



o 

 o 

 * 



