385 



Summary and Conclusion 



> 



1) The prime aim of thH txper iment was to explore the 

 effect o^ puff volume oo tSe tumonf enicity ol coo- 

 denute from a sundard ofarene. 



2) Three lime* per week from jSe ninth week o( IHe uniil 

 death female ASl »Cl-de<Tv«d alb«no mice were painted 

 oo their jKaved backs with corvdenute in acetone pro- 

 duced from a survdard cif arerte (SO) by Mason machines 

 at a rate of one puff per minute »nd puff voJumet of 



^0, 2S. artd SO mi. The condensate dose per animal ar>d 

 treatment was 50 mg . Each experWncnui group orifi- 

 naBjr induded 2S2 animah. 



3) The f>umbef of tumour-bearing animab in the condemate- 

 tTMted groups was 131 00 mJ). 170 (2S mi) and Kt 



(SO ml), seven of these m«ce exdusivYty showed tumours 

 whkh regressed. The proportioo of artimab with perma- 

 nent tumours rnferrt^ (o animab Mill alive at the 

 end of experirr>enul week 20 Oust before the fint 

 tumour occurrence) was practicany the same kn al 

 three groups (72.9, 73-5; 74.2 percent) due »o *fl^ 

 rent death rates. Higher lurrtortgefwary was recognised 

 by earlier tp^mnct of tumourv Utcreasing puff 

 volume reduced turrvxtgenicity from 10 ml via 2S mi to 

 SO ml. 



The number of animab with malignant tumours was 

 92 no tr^. 115 (25 ml) »rxi 122 (SO m\\ Onreganjing 

 the arMmab which died during the first 20 eiperi- 

 mental weeks, the percenuges of arumals with malig- 

 nancies were 52.0 (10 ml). S0.9 (2S ml) and 54J 

 (SO ml). With this correspondence berweeo groups. 

 the death rates after we«k 20 give *r\ indicaton of 

 (he se<)uerKe of tumortgeoKity for tht condensates, 

 whkh again demonstrates reduced carortogerMcity 

 with inaeasing puff volume. 



4) The rtumber of animab with permanent tumoon m the 

 SO mg group (puff volume 35 ml) of Eiperimeni BO was 

 17i. Related to the number of anirrvils alrve at the 



20th eipe<irr>enial week the inct^nce of tumour-beanr>g 

 antmah was 73 9 percent, that of animals with malig- 

 nar>oes S5 5 percem »vh»ch corresponds wuh ihe dau 

 of condensate groups m this Experiment Judged from 

 the ume the tumours occurred, the tumon|i«ntc effect 

 was siifhily higher than would be expecieo from the 

 resuhs of (hu Exper irr>ent assun-Mng rr>orK>tony-lype of 

 respome with puff volume. 



