115 



GENETIC CONCEPTS RELEVANT TO SUPPLEMENTATION 2 



1 . 1 Types of Genetic Risk 2 



1 .2 Genetic Processes Driving Genetic Risk 2 



1.3 Relationship Between Genetic Risk and Rate and Scale of 

 Supplementation 3 



BROODSTOCK COLLECT ION 4 



2.1 Choice of Donor Population for Use in Hatchery 

 Propagation ^ 



2.1.1 Restoration - priorities for choice of donor 

 population 5 



2.1.2 Augmentation- priorities for choice of donor 

 population ^ 



2.1.3 Augmentation of critically small populations- 

 special considerations 8 



2.1.4 Changes in priorities for donor stock sources 8 



2.2 Collecting Broodstock 



Years Before Hatchery Returns Are Present 8 



2.2.1. Collecting from moderate-sized populations 10 



2.2.2 Collecting from small populations 1 1 



2.2.3 Special attention to sex ratio 1 1 



2.2.4 Special attention to age structure 12 



2.3. Collecting Broodstock 



Years When Hatchery Returns Are Present 12 



2.4 Captive Broodstock Programs 14 



2.4.1 Captive broodstock program based on adult 

 collections 1^ 



2.4.2 Captive broodstock program based on juvenile 

 collections - 15 



2.5 Kelt Reconditioning (sieelhead only) 16 



SPAWNING PROTOCOLS 20 



3.1 Maximizing Effective Number 20 



3.1.1 Maximize Ne for hatchery broodstock in hand 20 



3.1.2 Maximize Ne for total h»u' population (Ryman and 

 Laikre issues) 20 



3.2 Mating Schemes - we will add figures to help explain 

 mating schemes 20 



3.2. 1 One by one 20 



3.2.1.1 Male use - single 21 



3.2.1.2 Male use - pair 21 



3.2.2 Factorial 2 1 



3.2.2.1 Least numerous sex - female, male use - 

 single 22 



