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in the prototype system. These will entail clinical signs of 

 stress such as plasma Cortisol, glucose and lactate. In 

 addition, we will ascertain the general health of the fish by 

 monitoring several features of the immune defense system. 

 Physical effects of the system will be determined by monitoring 

 descaling and mucus production. Mortality rates of fish having 

 experienced the system will be determined. 



We will evaluate several performances of test fish such as 

 seawater adaptability and swimming ability. Orientation 

 ability/migratory motivation will be established by tracking 

 radio-tagged individuals. Behavior of the fish in the pipe will 

 also be observed by video camera and other means. 



Attempts will be made to determine whether or not pathogens 

 may concentrate in the system. We will try to do this at a 

 hatchery where the fish range in BKD infections, using pipes to 

 hold small lots of fish for specified periods of time. 



Once trials have been conducted with spring chinook, we will 

 then evaluate fall chinook and systems also having the presence 

 of steelhead at various densities. Major trials will also have 

 to be repeated at different temperatures inherent to the Snake 

 and Columbia. All tests will have the appropriate number of 

 replications and test animals to allow for statistically 

 interpretable results. Hatchery fish will be used as test 

 animals because wild fish are unavailable for study. We 

 recognize that migrants (smolts) will have to be used for the 

 main evaluations. 



