the contents of vesicles where available, and where not 

 available, specimens from the freshest erosions or ulcers 

 should be forwarded. 



Inoculation of Test Animals. — In all suspicions in- 

 stances where a positive diagnosis can not be made, veteri- 

 narians should proceed immediately, without waiting for 

 a reply to their report, to make inoculation tests on hogs 

 or calves, preferably yearling calves, in order to ascertain 

 if the disease is transmissible . This may be done through 

 scarification of the gingiva of the test animal, into which 

 may be rubbed (with a clean cob or otlier swab) some of the 

 vesicular matter or specimens from lesions of the suspected 

 animal, or such inoculation may be made by the use of a 

 hypodermic syringe. 



Disinfection on Leaving Premises. — Care should be 

 taken on leaving any suspected premises to disinfect the 

 person and clothing in the manner described in another 

 chapter. 



PROCEDURE WHERE INFECTION IS FOUND. 



Cooperation with State Authorities. — As soon as 

 the diagnosis has been confirmed by the special represent- 

 ative of the department, the chief of the bureau will invite 

 the proper State authorities to cooperate with the depart- 

 ment in the arrest and eradication of the disease, and a 

 veterinary inspector will be designated to take charge of 

 the work. The latter should promptly ask for the neces- 

 sary help, and cooperate with the State authorities in ascer- 

 taining the extent of the outbreak and in formulating State 

 quarantine regulations. He should prevail upon the State 

 authorities to quarantine the infected premises immedi- 

 ately, and endeavor to make arrangements with them to 

 place guards on each of the infected premises day and night 

 to see that the quarantine is preserved. 



Quarantine. — The owners of affected herds should be 

 fully instructed concerning the nature of the disease and 



