EFFECT OF THYROIDECTOMY ON NITROGENOUS METABOLISM. 479 



The animals during observation were kept in chambers with 

 glass sides and an earthenware floor sloping towards one 

 corner, where a funnel-shaped aperture conveyed the urine 

 to a basin placed beneath. Two chambers were used for each 

 dog, being used on alternate days. After changing, the first 

 chamber was washed out, and the washings kept for estima- 

 tion of the nitrogen in them. The faeces were collected and 

 weighed daily. A measured quantity of water was given each 

 day, so that the amount drank was determined. Weighed 

 quantities of dog biscuit were given. 



The amount of nitrogen present in food, urine and ft^ces was 

 determined by Kjeldahl's method. Urea was estimated by the 

 hypobromite method, total solids by evaporation at 80" in a 

 platinum dish. Total sulphates were estimated by precipi- 

 tating 50 C.C. of urine with a mixture of BaCl2 and Ba (0H)2 

 after acidifying with HCl _ then boiling. " The aromatic 

 sulphates were determined in 100 c.c. by precipitating with 

 barium mixture, filtering, acidifying with HCl, boiling, and 

 then placing the boiled filtrate in a hot oven for one or two 

 hours. 



The blood for analysis was obtained by inserting a canula 

 in the carotid under chloroform. The urea and nitrogen 

 estimations in the tissues were made from extracts with 

 absolute alcohol and with rectified spirit. 



Bccord of Experiments. 

 No. I. 



Small bitch. Wt., 4-5 kilos. 

 Oct. 5. — Put in chamber. 



* This was in the washings of the chamber. 

 VOL. XXXV. (n.S. vol. XV.)— JULY 1901. 



2 K 



