110 Manual of Veterinary Microbiology. 



The solutions most used are those of gentian violet, 

 methyl violet, fuclisin, and methylene blue. A few- 

 minutes contact is sufficient : five for cover glasses, 

 fifteen for sections, a little longer in the case of 

 methylene blue. 



Lofflers method. — Cover glasses are stained for from 

 five to ten minutes in the following medium : 



Solution of caustic potash, 1 to 10,000, 3 cub. cent. 



Saturated alcoholic solution of methyl- 

 ene blue, . . . . . 1 " " 



All the elements are stained deep blue. To bring 

 out the microbes, the stained preparations are im- 

 mersed in w^ater containing a small quantity of acetic 

 acid, one or two drops to a watch glass; in this they 

 remain on an average only one minute; they are then 

 washed with distilled water and mounted. The mi- 

 crobes appear colored a deej) blue, the tissue ele- 

 ments a light blue. 



Method of Malassez and Vignal. — Staining is here 

 made with Malassez' blue, which consists of : 



Aniline water, .... 9 cub. cent. 



Absolute alcohol, . . . . 1 " " 



Saturated alcoholic solution of methyl- 

 ene blue, 1 u « 



Sections remain therein ten minutes, cover glasses 

 five minutes ; they are then decolorized in the follow- 

 ing mixture : 



Absolute alcohol, . . . .1 cub. cent. 



2 per cent solution of sodium carbonate, 2 " " 



Methods of double staining. 

 Gram's method. — Gram's violet contains: 

 Aniline water, .... 10 cub. cent. 



