142 Manual of Veterinary, 3Iicrobiology. 



Inoculations may be made in any region, but, 

 preferably, in places where the skin is thin and pliant 

 (internal face of the thighs, abdominal wall, pectoral 

 region in birds), and the celhilar tissue abundant. 



3. Intra-peritoneal inoculations. — In order to intro- 

 duce the virulent matter into the abdomen, the ani- 

 mal being held well on the back, the abdominal walls 

 are pinched up between the thumb and index, and 

 the operation continued as above, after being well as- 

 sured that the point of the syringe is indeed free 

 within the abdomen. It is necessary here to operate 

 with a certain amount of care in order to avoid 

 wounding the viscera an accident especially to be 

 feared in birds. 



4. Intra-venous inoculation. — This method necessi- 

 tates the employment of a liquid free from solid par- 

 ticles which, by their arrest in the smaller vessels, 

 might occasion fatal embolisms. In performing this 

 inoculation the vein is distended by pressure exerted 

 on its course and the canula introduced into it, the 

 point directed toward the heart ; the operation is suc- 

 cessful when a drop of blood issues at the shoulder 

 of the canula; a graduated pressure is then brought 

 to bear on the piston. 



For this inoculation the most salient superficial 

 veins are selected, in the rabbit the veins of the ear, 

 in birds the vein of the arm. In some animals the in- 

 oculation can be made into the jugular, saphena, etc. 



5. Inoculations in the anterior chamber of the eye. — 

 The eye is first ansesthized by means of a few drops 

 of a solution of cocaine, at 1 to 20; then, the globe 

 beinsf immobilized, the canula is insinuated horizon- 

 tally through the cornea in its eccentric part. One 



