186 



Manual of Veterinary Microbiology. 



Fig. 6. 



readily takes the difierent aniline stains ; the blood 

 may be stained with aqueous solutions either directly 



or after being dried 

 on the cover glass ; 

 in this last case the 

 bacteridia are gene- 



V '^fcaS™* ^ B rally shorter and 



^^^M^^*.^^ %^ ^^ more slender. The 



double stains of 

 Gram and Weigert 

 give excellent re- 

 sults.* 



Examination of 

 fresh blood is espe- 

 cially to be recom- 

 mended here, as it 

 enables us to estab- 

 lish the immobility 

 of the bacilli and the 

 alterations of the blood corpuscles. In examining 

 the blood of a carcass for bacteridia the specimen 

 must be taken from a deep vein some distance from 

 the peritoneum, because, in this disease, the invasion 

 of microbes coming from the abdominal viscera is 

 more rapid as the charbon bacillus, being aerobic, has 

 deprived the blood of its oxygen. 



Experimental inoculations. — Inoculation^ may be 

 made by subcutaneous injection, by intra-vascular 



* [The Gram stain is especially applicable to the staining of sec- 

 tions. On cover-glass preparations the characteristic appearance 

 of the bacillus is best brought out by the use of aqueous or hydro- 

 alcoholic solutions. Loffler's stain, followed or preceded by eosin, 

 gives excellent results. — D.] 



Anthrax bacilli in the blood. 

 ( Cover-glass preparation ) . — Kitt. 



