198 Manual of Veterinary Microbiology. 



artificial culture ; it becomes obliterated in four or five 

 transfers. 



The material for inoculation of culture media maybe 

 taken from the juice obtained by scraping the center 

 of a tumor, from the peritoneal serosity, and from the 

 blood ; but they only appear in this last fluid after 

 death ; as they are present in small numbers it is well 

 to allow them to multiply by keeping a quantity of 

 this blood in the incubator for twenty-four hours. 



Research and coloration. — The bacillus Chauvoei ex- 

 ists in great abundance in the muscular tumor, sus- 

 pended in the fluid with which it is infiltrated and 

 which is interposed between the contractile elements. 

 In the last moments of life and after death it is also met 

 with in small numbers in the blood ; finally, it exists in 

 abundance in the bile and in the peritoneal serosity. 



Simple methods of staining are alone successful; 

 the various hydro-alcoholic solutions are available for 

 this purpose, but Loffler's method should be preferred 

 for sections. 



Experimental inoculations. — The species to which it 

 is possible to communicate the experimental disease 

 are the ox, sheep, goat, and guinea pig. The rabbit is 

 refractory. The ass and the horse only contract a local 

 engorgement. 



The receptivity of the various species for this bacillus 

 sufiiciently difierentiates it from that of Pasteur's sep- 

 ticsemia which it much resembles in its physical char- 

 acters, staining proclivities, and its anaerobic faculty, 

 and in the emphysemato-gangrenous lesions which it 

 occasions. The septic bacillus is pathogenic for all 

 species except the ox ; the bacillus of symptomatic 



