Microhic Diseases Individually Considered. 205 



show any specific pathogenic property. This last 

 germ is never seen in the experimental disease and 

 probably comes from a secondary invasion occurring 

 in the diseased animal. The point of departure of 

 this collateral infection appears to be the intestine, in 

 which the bacillus just mentioned is found in abun- 

 dance; it is present in the blood in smaller numbers 

 the further removed the latter is from the abdominal 

 cavity. 



Pasteur and Tliuillier, who first described the bacil- 

 lus of rouget, describe it as of a figure 8 form, but 

 this was an error of observation which our staining 

 methods and improved instruments have corrected. 



The bacillus of rouget is non-motile. 



Cultures. — The bacillus of rouget is especially ana- 

 erobic but it also grows in contact with the air ; it 

 multiplies at a temperature as low as 20°, but grows 

 especially well in the incubator. 



The blood and the pulp of the various diseased or- 

 gans may be used for the inoculation of culture media. 

 This material will more likely be pure when taken 

 from a part at some distance from the abdominal cav- 



The germ grows well on the various culture media : 

 on potato, especially in presence of oxygen, the growth 

 is feeble. 



In bouillon it produces, after forty-eight hours, a 

 slight uniform turbidity, which afterward becomes de- 

 posited as a whitish gray sediment. 



Stab cultures in gelatin are characteristic. The 

 germ multiplies especially in the deeper parts and 

 forms along the course of the puncture a track from 

 which radiate silky tufts, giving to the whole the as- 



